{"id":3512,"date":"2024-08-02T14:18:37","date_gmt":"2024-08-02T14:18:37","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=3512"},"modified":"2024-08-02T14:18:37","modified_gmt":"2024-08-02T14:18:37","slug":"chamagu-isothiocyanate","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/chamagu-isothiocyanate\/","title":{"rendered":"A Chamagu izotiocian\u00e1t enzimatikus el\u0151\u00e1ll\u00edt\u00e1sa \u00e9s m\u00e9hnyakr\u00e1kellenes aktivit\u00e1sa"},"content":{"rendered":"

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"<\/p>\n

A Chamagu izotiocian\u00e1t enzimatikus el\u0151\u00e1ll\u00edt\u00e1sa \u00e9s m\u00e9hnyakr\u00e1kellenes aktivit\u00e1sa<\/p>\n

Az ut\u00f3bbi \u00e9vekben a r\u00e1k el\u0151fordul\u00e1si ar\u00e1nya gyorsan n\u00f6vekszik K\u00edn\u00e1ban, \u00e9s a r\u00e1k megel\u0151z\u00e9se \u00e9s kezel\u00e9se s\u00fcrg\u0151s. A kutat\u00e1sok azt mutatj\u00e1k, hogy a keresztesvir\u00e1g\u00fa z\u00f6lds\u00e9gek fogyaszt\u00e1s\u00e1nak n\u00f6vel\u00e9se hat\u00e9konyan cs\u00f6kkentheti a r\u00e1k el\u0151fordul\u00e1si ar\u00e1ny\u00e1t. A keresztesvir\u00e1gzat\u00fa z\u00f6lds\u00e9gek b\u0151s\u00e9gesen tartalmaznak gl\u00fckozinol\u00e1tokat, m\u00e1s n\u00e9ven gl\u00fckozinol\u00e1tokat (GL). Amikor a n\u00f6v\u00e9nyek mechanikusan k\u00e1rosodnak, a GL-eket a saj\u00e1t mirozin\u00e1zuk hidroliz\u00e1lja izotiocian\u00e1tok el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra. Az izotiocian\u00e1tok a konjug\u00e1lt kett\u0151s k\u00f6t\u00e9seket tartalmaz\u00f3 vegy\u00fcletek oszt\u00e1lya, molekul\u00e1ris k\u00e9plet\u00fck R-N=C=S (az R k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa alkilcsoportokat jelent). Az ut\u00f3bbi \u00e9vekben az izotiocian\u00e1tok kiemelked\u0151 biol\u00f3giai aktivit\u00e1suk miatt a kutat\u00e1sok f\u00f3kusz\u00e1ba ker\u00fcltek. A kutat\u00e1sok kimutatt\u00e1k, hogy az izotiocian\u00e1tok k\u00fcl\u00f6nb\u00f6z\u0151 biol\u00f3giai aktivit\u00e1sokkal rendelkeznek, mint p\u00e9ld\u00e1ul daganatellenes, antibakteri\u00e1lis, szabadgy\u00f6k-fog\u00f3 \u00e9s k\u00f3rokoz\u00f3val szembeni ellen\u00e1ll\u00e1s. Sz\u00e1mos ter\u00fcleten, p\u00e9ld\u00e1ul az eg\u00e9szs\u00e9g\u00fcgyben, az \u00e9lelmiszeriparban \u00e9s a mez\u0151gazdas\u00e1gban fejlesztett\u00e9k ki \u00e9s haszn\u00e1lj\u00e1k.<\/p>\n

A Brassica rapa L., tudom\u00e1nyos nev\u00e9n \"feh\u00e9rr\u00e9pa\", a Brassicaceae csal\u00e1dba tartoz\u00f3 k\u00e9t\u00e9ves l\u00e1gysz\u00e1r\u00fa n\u00f6v\u00e9ny. F\u0151leg a Tianshan-hegys\u00e9g d\u00e9lnyugati r\u00e9sz\u00e9n \u00e9s a Tarimt\u00f3l \u00e9szaknyugatra, Hszincsiangban elterjedt, \u00e9s helyi z\u00f6lds\u00e9gk\u00fcl\u00f6nlegess\u00e9g, amelyet Hszincsiangban minden etnikai csoporthoz tartoz\u00f3 emberek \u00e1ltal\u00e1nosan fogyasztanak. A kutat\u00e1sok meg\u00e1llap\u00edtott\u00e1k, hogy a chamagu gy\u00f6kerek b\u0151s\u00e9ges GL-t tartalmaznak. Zhuang \u00e9s munkat\u00e1rsai ultra-nagy folyad\u00e9kkromatogr\u00e1fi\u00e1s technol\u00f3gi\u00e1t alkalmaztak a Xinjiang r\u00e9pa k\u00fcl\u00f6nb\u00f6z\u0151 fejl\u0151d\u00e9si szakaszaiban \u00e9s n\u00f6veked\u00e9si r\u00e9szeiben (gy\u00f6kerek, sz\u00e1rak, levelek) tal\u00e1lhat\u00f3 GL-ek t\u00edpusainak \u00e9s tartalm\u00e1nak elemz\u00e9s\u00e9re a t\u00e1pl\u00e1lkoz\u00e1si n\u00f6veked\u00e9si id\u0151szakban. Meg\u00e1llap\u00edtott\u00e1k, hogy a gl\u00fckozinol\u00e1tok t\u00edpusai \u00e9s t\u00f6megfrakci\u00f3i k\u00f6z\u00f6tt bizonyos k\u00fcl\u00f6nbs\u00e9gek voltak a t\u00e1pn\u00f6veked\u00e9s k\u00fcl\u00f6nb\u00f6z\u0151 fejl\u0151d\u00e9si szakaszaiban. A n\u00f6v\u00e9nyekben a GL-ek t\u00edpusainak \u00e9s tartalmainak k\u00fcl\u00f6nbs\u00e9gei az izotiocian\u00e1tok t\u00edpusainak \u00e9s tartalmainak elt\u00e9r\u00e9seihez vezetnek, ami k\u00fcl\u00f6nb\u00f6z\u0151 biol\u00f3giai aktivit\u00e1sokat eredm\u00e9nyez. Sun arr\u00f3l sz\u00e1molt be, hogy a Qiamagu magvak n\u00e9gyf\u00e9le izotiocian\u00e1tot tartalmaznak: fenetil-izotiocian\u00e1tot, tert-butil-izotiocian\u00e1tot, izopropil-izotiocian\u00e1tot \u00e9s allil-izotiocian\u00e1tot.<\/p>\n

Jelenleg az izotiocian\u00e1tok el\u0151\u00e1ll\u00edt\u00e1sa f\u0151k\u00e9nt k\u00e9miai szint\u00e9zist \u00e9s enzimatikus hidrol\u00edzist foglal mag\u00e1ban. Ezek k\u00f6z\u00fcl a k\u00e9miai szint\u00e9zis m\u00f3dszere bonyolult l\u00e9p\u00e9sekkel, sok k\u00f6ztes term\u00e9kkel j\u00e1r, nem k\u00f6rnyezetbar\u00e1t \u00e9s alacsony a szint\u00e9zis sebess\u00e9ge; az enzimatikus hidrol\u00edzis a keresztesvir\u00e1g\u00fa n\u00f6v\u00e9nyek gl\u00fckozinol\u00e1t-mirozin\u00e1z rendszer\u00e9t haszn\u00e1lja az izotiocian\u00e1tok kivon\u00e1s\u00e1ra, amelynek el\u0151nye az egyszer\u0171 m\u0171k\u00f6d\u00e9s \u00e9s a viszonylag magas hozam. Az enzimatikus hidrol\u00edzist endog\u00e9n mirozin\u00e1z lebont\u00e1si m\u00f3dszerre \u00e9s exog\u00e9n mirozin\u00e1z lebont\u00e1si m\u00f3dszerre osztj\u00e1k. Az endog\u00e9n enzimatikus hidrol\u00edzis alkalmaz\u00e1sa az izotiocian\u00e1tok el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra, b\u00e1r a folyamat egyszer\u0171, a n\u00f6v\u00e9nyekben l\u00e9v\u0151 endog\u00e9n mirozin\u00e1z nem tud teljesen felszabadulni, ami hossz\u00fa enzimatikus hidrol\u00edzis id\u0151t \u00e9s alacsony hat\u00e9konys\u00e1got eredm\u00e9nyez. Ding \u00f6sszehasonl\u00edtotta a repc\u00e9ben az endog\u00e9n \u00e9s exog\u00e9n enzimek \u00e1ltal v\u00e9gzett GL-lebont\u00e1s sor\u00e1n keletkez\u0151 izotiocian\u00e1tok mennyis\u00e9g\u00e9t, \u00e9s meg\u00e1llap\u00edtotta, hogy az exog\u00e9n mirozin\u00e1z \u00e1ltal termelt izotiocian\u00e1tok mennyis\u00e9ge 7,6-szorosa volt az endog\u00e9n mirozin\u00e1z\u00e9nak. A GC-MS azonos\u00edt\u00e1s \u00e9s elemz\u00e9s azt mutatta, hogy az exog\u00e9n mirozin\u00e1znak sokf\u00e9le boml\u00e1sterm\u00e9ke volt, viszonylag magas tartalommal \u00e9s kevesebb szennyez\u0151d\u00e9ssel.<\/p>\n

Ebben a tanulm\u00e1nyban a Chamagu gy\u00f6keret haszn\u00e1lt\u00e1k nyersanyagk\u00e9nt, \u00e9s exog\u00e9n mirozin\u00e1z lebont\u00e1si m\u00f3dszert alkalmaztak a Chamagu izotiocian\u00e1t (BRICe) el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz. Az egyt\u00e9nyez\u0151s sz\u0171r\u00e9s alapj\u00e1n az enzimhidrol\u00edzis ideje, a h\u0151m\u00e9rs\u00e9klet, a pH \u00e9s az aszkorbinsav koncentr\u00e1ci\u00f3ja jelent\u0151s befoly\u00e1sol\u00f3 t\u00e9nyez\u0151k\u00e9nt azonos\u00edtott\u00e1k. A kivon\u00e1si folyamat optimaliz\u00e1l\u00e1s\u00e1ra n\u00e9gyfaktoros h\u00e1romszint\u0171 ortogon\u00e1lis k\u00eds\u00e9rletet alkalmaztunk. V\u00e9g\u00fcl a k\u00e9miai \u00f6sszet\u00e9telt g\u00e1zkromatogr\u00e1fia-t\u00f6megspektrometri\u00e1val (GC-MS) elemezt\u00e9k, \u00e9s tanulm\u00e1nyozt\u00e1k az izotiocian\u00e1t r\u00e1kellenes hat\u00e1s\u00e1t.<\/p>\n

A r\u00e1k az egyik olyan rosszindulat\u00fa betegs\u00e9g, amely az emberi \u00e9let biztons\u00e1g\u00e1t fenyegeti. T\u00f6bb j\u00e1rv\u00e1nytani vizsg\u00e1lat meg\u00e1llap\u00edtotta, hogy t\u00f6bb keresztesvir\u00e1g\u00fa z\u00f6lds\u00e9g fogyaszt\u00e1sa hat\u00e9konyan cs\u00f6kkentheti a r\u00e1k kock\u00e1zat\u00e1t. Az izotiocian\u00e1t a keresztesvir\u00e1g\u00fa z\u00f6lds\u00e9gek \u00f6nenzimatikus hidrol\u00edzis\u00e9nek egyik term\u00e9ke. A kutat\u00e1sok kimutatt\u00e1k, hogy az izotiocian\u00e1tok k\u00e9pesek aktiv\u00e1lni a II. f\u00e1zis\u00fa enzimeket az emberi szervezetben, lehet\u0151v\u00e9 t\u00e9ve az I. f\u00e1zis\u00fa reakci\u00f3k term\u00e9keinek a gl\u00fckuronsavhoz \u00e9s a glutationhoz val\u00f3 k\u00f6t\u0151d\u00e9s\u00e9t, ez\u00e1ltal el\u0151seg\u00edtve a r\u00e1kkelt\u0151 anyagok gyors ki\u00fcr\u00fcl\u00e9s\u00e9t a szervezetb\u0151l. Ez\u00e9rt az izotiocian\u00e1tok r\u00e1kg\u00e1tl\u00f3k\u00e9nt haszn\u00e1lhat\u00f3k a gy\u00f3gy\u00e1szatban. A Qiamagu egy gy\u00f3gy- \u00e9s ehet\u0151 n\u00f6v\u00e9ny Hszincsiangban, amely k\u00fcl\u00f6nb\u00f6z\u0151 hat\u00f3anyagokat, p\u00e9ld\u00e1ul gl\u00fckozinol\u00e1tokat, flavonoidokat, izotiocian\u00e1tokat \u00e9s poliszacharidokat tartalmaz. A Qiamagu izotiocian\u00e1tok fejleszt\u00e9se \u00e9s felhaszn\u00e1l\u00e1sa azonban jelenleg el\u00e9gtelen. Az enzimatikus hidrol\u00edzis jelenleg k\u00f6rnyezetbar\u00e1t m\u00f3dszer az izotiocian\u00e1tok kivon\u00e1s\u00e1ra. Az enzimatikus hidrol\u00edzis k\u00f6r\u00fclm\u00e9nyeinek optimaliz\u00e1l\u00e1s\u00e1val nemcsak az egy\u00e9b m\u00e1sodlagos metabolitok termel\u00e9se cs\u00f6kkenthet\u0151, hanem a c\u00e9lterm\u00e9k termel\u00e9si sebess\u00e9ge is felgyors\u00edthat\u00f3, ez\u00e1ltal n\u00f6velhet\u0151 a hozam. Jang a Brassicaceae csal\u00e1dban endog\u00e9n mirozin\u00e1zzal \u00e1ll\u00edtotta el\u0151 az izotiocian\u00e1tokat. B\u00e1r az endog\u00e9n enzimes m\u00f3dszer egyszer\u0171 folyamat, a n\u00f6v\u00e9nyekben l\u00e9v\u0151 mirozin\u00e1z nem tud teljesen felszabadulni, ami hossz\u00fa enzimes hidrol\u00edzisid\u0151t \u00e9s alacsony hat\u00e9konys\u00e1got eredm\u00e9nyez. Az endog\u00e9n enzimekkel \u00f6sszehasonl\u00edtva, az exog\u00e9n enzimek haszn\u00e1lata az izotiocian\u00e1tok el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz k\u00f6nnyebben szab\u00e1lyozhat\u00f3 az exog\u00e9n enzimek enzimatikus hidrol\u00edzis\u00e9nek k\u00f6r\u00fclm\u00e9nyei, ami el\u0151ny\u00f6s az enzimatikus hidrol\u00edzis term\u00e9keinek t\u00edpusaiban bek\u00f6vetkez\u0151 v\u00e1ltoz\u00e1sok ir\u00e1ny\u00edt\u00e1sa \u00e9s a term\u00e9kek mennyis\u00e9g\u00e9nek n\u00f6vel\u00e9se szempontj\u00e1b\u00f3l. Ez\u00e9rt az izotiocian\u00e1tok el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak kulcsa a k\u00fcls\u0151 enzimes hidrol\u00edzis felt\u00e9teleinek optimaliz\u00e1l\u00e1sa.<\/p>\n

Az enzimatikus hidrol\u00edzisterm\u00e9kek k\u00e9pz\u0151d\u00e9se nemcsak a gl\u00fckozinol\u00e1t t\u00edpus\u00e1val f\u00fcgg \u00f6ssze, hanem mag\u00e1ban foglalja a hidrol\u00edzis idej\u00e9t, a hidrol\u00edzis h\u0151m\u00e9rs\u00e9klet\u00e9t, a pH-t, a nyom\u00e1st \u00e9s a Fe2+ jelenl\u00e9t\u00e9t is. Latt \u00e9 meg\u00e1llap\u00edtotta, hogy ha az oldat pH-ja semleges, akkor a gl\u00fckozinol\u00e1tok sz\u00e1m\u00e1ra kedvez\u0151 az izotiocian\u00e1tok k\u00e9pz\u0151d\u00e9se. Ha azonban a pH savas\/l\u00fagos, vagy ha ciklikus k\u00e9nre jellemz\u0151 feh\u00e9rj\u00e9k vagy Fe2+ van jelen, az enzimatikus hidrol\u00edzis term\u00e9kei nagy mennyis\u00e9g\u0171 nitrilt k\u00e9peznek. Yang v\u00e1laszfel\u00fcleti m\u00f3dszert haszn\u00e1lt a pH, az EDTA \u00e9s az aszkorbinsav t\u00e9nyez\u0151inek elemz\u00e9s\u00e9re, \u00e9s meg\u00e1llap\u00edtotta, hogy ez a h\u00e1rom t\u00e9nyez\u0151 jelent\u0151s hat\u00e1ssal van az izotiocian\u00e1t extrakci\u00f3s mennyis\u00e9g\u00e9re. Burmeister meg\u00e1llap\u00edtotta, hogy az aszkorbinsav helyettes\u00edtette a katalitikus b\u00e1zis funkci\u00f3t a mirozin\u00e1z akt\u00edv hely\u00e9n, a mirozin\u00e1z katalitikus tulajdons\u00e1gait mutatva. Megfelel\u0151 mennyis\u00e9g\u0171 aszkorbinsav hozz\u00e1ad\u00e1sa a friss brokkolihoz jelent\u0151sen fokozhatja a mirozin\u00e1z aktivit\u00e1s\u00e1t, ez\u00e1ltal n\u00f6velve az izotiocian\u00e1tok termel\u00e9s\u00e9t. Ez a tanulm\u00e1ny exog\u00e9n (feh\u00e9r must\u00e1rmagb\u00f3l sz\u00e1rmaz\u00f3) mirozin\u00e1z lebont\u00e1si m\u00f3dszert alkalmazott a BRIe el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz. Az enzimatikus hidrol\u00edzis k\u00f6r\u00fclm\u00e9nyeit egyt\u00e9nyez\u0151s \u00e9s ortogon\u00e1lis k\u00eds\u00e9rletekkel optimaliz\u00e1ltuk, \u00e9s n\u00e9gy olyan t\u00e9nyez\u0151t vizsg\u00e1ltunk, amelyek jelent\u0151s hat\u00e1ssal voltak a BRIe kivon\u00e1s\u00e1ra: az enzimatikus hidrol\u00edzis ideje, az enzimatikus hidrol\u00edzis h\u0151m\u00e9rs\u00e9klete, az aszkorbinsav koncentr\u00e1ci\u00f3ja \u00e9s a pH. A BRIe optim\u00e1lis enzimatikus hidrol\u00edzisfelt\u00e9teleit a k\u00f6vetkez\u0151k\u00e9ppen hat\u00e1rozt\u00e1k meg: 20 \u03bc L mirozin\u00e1z hozz\u00e1ad\u00e1sa, 70 \u2103 reakci\u00f3h\u0151m\u00e9rs\u00e9klet, 3 \u00f3ra reakci\u00f3id\u0151, 6,5 pH, 0,1 mg\/g aszkorbinsav-koncentr\u00e1ci\u00f3 \u00e9s 2 g GLs fagyasztva sz\u00e1r\u00edtott por. \u00c9rdekes, hogy az optim\u00e1lis enzimatikus hidrol\u00edzis h\u0151m\u00e9rs\u00e9klete ebben a vizsg\u00e1latban 70 \u2103. Vannak kutat\u00e1si jelent\u00e9sek, amelyek szerint a brokkolib\u00f3l sz\u00e1rmaz\u00f3 n\u00f6v\u00e9nyi izotiocian\u00e1tok enzimatikus hidrol\u00edzis\u00e9nek h\u0151m\u00e9rs\u00e9kleti tartom\u00e1nya 20-60 \u2103, az optim\u00e1lis enzimatikus hidrol\u00edzis h\u0151m\u00e9rs\u00e9klete 40 \u2103; A wasabi gy\u00f6kerekb\u0151l sz\u00e1rmaz\u00f3 izotiocian\u00e1tok kivon\u00e1s\u00e1nak h\u0151m\u00e9rs\u00e9kleti tartom\u00e1nya 30-90 \u2103, az optim\u00e1lis enzimatikus hidrol\u00edzis h\u0151m\u00e9rs\u00e9klete 70 \u2103. A mirozin\u00e1z optim\u00e1lis h\u0151m\u00e9rs\u00e9klete teh\u00e1t a sz\u00e1rmaz\u00e1si fajt\u00f3l f\u00fcgg\u0151en v\u00e1ltozik.<\/p>\n

A k\u00e9pz\u0151d\u0151 izotiocian\u00e1tok t\u00edpusai \u00e1ltal\u00e1ban az enzimatikus hidrol\u00edzis k\u00f6r\u00fclm\u00e9nyeit\u0151l f\u00fcgg\u0151en v\u00e1ltoznak. Chen et al. endog\u00e9n enzimatikus hidrol\u00edzist haszn\u00e1lt, hogy a brokkoliban az enzimatikus hidrol\u00edzis term\u00e9keik\u00e9nt alil-izotiocian\u00e1tot, izobutil-izotiocian\u00e1tot, 1-butenil-izotiocian\u00e1tot, 4-metil-tionitrilt azonos\u00edtsa, 5-metilti\u00f3pentonitril, 3-(metiltio-)propil-izotiocian\u00e1t, butil-izotiocian\u00e1t, retek-tiocian\u00e1t \u00e9s retek-szulforaf\u00e1n GC-MS seg\u00edts\u00e9g\u00e9vel, a retek-szulforaf\u00e1n tartalma volt a legmagasabb. Yang exog\u00e9n enzimatikus hidrol\u00edzist alkalmazott, hogy a brokkoliban l\u00e9v\u0151 enzimatikus hidrol\u00edzis term\u00e9kei az alil-izotiocian\u00e1t, fenetil-izotiocian\u00e1t, ciklopentil-izotiocian\u00e1t, fenilpropanonitril, 2,1-feniloxazolidinon \u00e9s pajzsmirigy-stimul\u00e1l\u00f3 hormon. Ez a tanulm\u00e1ny GC-MS seg\u00edts\u00e9g\u00e9vel elemezte a BRIe optim\u00e1lis enzimatikus hidrol\u00edzisfelt\u00e9teleinek \u00f6sszet\u00e9tel\u00e9t, \u00e9s h\u00e9t enzimatikus hidrol\u00edzishez kapcsol\u00f3d\u00f3 term\u00e9ket azonos\u00edtott, nevezetesen 3-amino-2-oxazolidinon, 2-oxazolidinetion, 3-buten-1-ilizotiocian\u00e1t (BITC), fenilizotiocian\u00e1t (PEITC), 1-izotiocian\u00e1t-propil\u00e9szter, izotiocian\u00e1t-izopropil-\u00e9szter \u00e9s 2-fenilizotiocian\u00e1t (2-PITC), amelyek k\u00f6z\u00fcl 4 izotiocian\u00e1t t\u00edpus volt.<\/p>\n

Sz\u00e1mos tanulm\u00e1ny kimutatta, hogy az izotiocian\u00e1tok megel\u0151zhetik a k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e1kos megbeteged\u00e9sek kialakul\u00e1s\u00e1t. A kutat\u00e1sok meg\u00e1llap\u00edtott\u00e1k, hogy a PEITC d\u00f3zisf\u00fcgg\u0151en g\u00e1tolja az eg\u00e9r K7M2 csontritkul\u00e1sos sejtvonal prolifer\u00e1ci\u00f3j\u00e1t, \u00e9s G2\/M sejtciklus-meg\u00e1ll\u00e1st \u00e9s apopt\u00f3zist okozhat; a BITC jelent\u0151sen cs\u00f6kkenti a gyomor adenokarcin\u00f3ma sejt AGS sejtj\u00e9nek \u00e9letk\u00e9pess\u00e9g\u00e9t, \u00e9s apopt\u00f3zist \u00e9s autof\u00e1gi\u00e1t induk\u00e1l. Az izotiocian\u00e1tok m\u00e9hnyakr\u00e1kra gyakorolt hat\u00e1sair\u00f3l \u00e9s mechanizmusair\u00f3l azonban jelenleg kev\u00e9s jelent\u00e9s \u00e1ll rendelkez\u00e9sre. Ez a vizsg\u00e1lat egy in vitro sejtmodell seg\u00edts\u00e9g\u00e9vel meg\u00e1llap\u00edtotta, hogy a BRIe jelent\u0151sen g\u00e1tolta a Hela, Siha \u00e9s U14 m\u00e9hnyakr\u00e1ksejtek prolifer\u00e1ci\u00f3j\u00e1t id\u0151- \u00e9s d\u00f3zisf\u00fcgg\u0151 m\u00f3don. E h\u00e1rom sejtt\u00edpus 24 \u00f3r\u00e1s IC50 \u00e9rt\u00e9keit \u00f6sszehasonl\u00edtva arra a k\u00f6vetkeztet\u00e9sre jutottunk, hogy a Hela sejtek a leg\u00e9rz\u00e9kenyebbek a BRIe-re. A kontroll\u00e1latlan sejtprolifer\u00e1ci\u00f3 a r\u00e1k alapvet\u0151 oka, ami a sejtciklus megszakad\u00e1s\u00e1nak k\u00f6sz\u00f6nhet\u0151. Az \u00e1raml\u00e1si citometri\u00e1s elemz\u00e9s kimutatta, hogy a BRIe k\u00e9pes meg\u00e1ll\u00edtani a Hela sejtciklust az S \u00e9s G2\/M f\u00e1zisban. A r\u00e1kos sejtek \u00e1tt\u00e9tk\u00e9pz\u00e9se mindig is a kutat\u00e1s egyik forr\u00f3 pontja \u00e9s a klinikai kezel\u00e9s kih\u00edv\u00e1sa volt. Karcol\u00e1sos k\u00eds\u00e9rletekkel meg\u00e1llap\u00edtott\u00e1k, hogy a BRIe hat\u00e9konyan g\u00e1tolja a Hela sejtek migr\u00e1ci\u00f3j\u00e1t. A Hochest 33258 fest\u00e9s kimutatta, hogy a BRIe k\u00e9pes DNS kondenz\u00e1ci\u00f3t induk\u00e1lni a Hela sejtmagokban, \u00e9s l\u00e1that\u00f3 szemcs\u00e9s fluoreszcencia figyelhet\u0151 meg a sejtmagokban, ami arra utal, hogy ez egy apoptotikus test. Tov\u00e1bbi elemz\u00e9s Annexin V-FITC kett\u0151s fest\u00e9ssel kimutatta, hogy a BRIe-vel kezelt Hela sejtek apopt\u00f3zis\u00e1nak ar\u00e1nya 24 \u00f3ra eltelt\u00e9vel a koncentr\u00e1ci\u00f3 n\u00f6veked\u00e9s\u00e9vel n\u0151tt, ami azt jelzi, hogy a BRIe apopt\u00f3zist induk\u00e1l a Hela sejtekben. \u00d6sszefoglalva, a BRIe jelent\u0151s m\u00e9hnyakr\u00e1kellenes aktivit\u00e1st mutat. A GC-MS adatok alapj\u00e1n felt\u00e9telezhet\u0151, hogy a BITC, a PEITC, az izopropil-izotiocian\u00e1t \u00e9s a 2-PITC lehetnek a f\u0151 hat\u00f3anyagai, amelyeket in vitro \u00e9s in vivo k\u00eds\u00e9rletekkel tov\u00e1bb kell valid\u00e1lni.<\/p>\n

\u00d6sszefoglalva, ez a tanulm\u00e1ny el\u0151sz\u00f6r hat\u00e1rozta meg az izotiocian\u00e1tok exog\u00e9n enzimes hidrol\u00edzis\u00e9nek optim\u00e1lis el\u0151k\u00e9sz\u00edt\u00e9si folyamat\u00e1t a Chamagu-ban. H\u00e9t enzimatikus hidrol\u00edzishez kapcsol\u00f3d\u00f3 term\u00e9ket azonos\u00edtottunk GC-MS anal\u00edzis seg\u00edts\u00e9g\u00e9vel, \u00e9s a BRIe enzimatikus hidrol\u00edzisb\u0151l nyert term\u00e9k k\u00e9pes g\u00e1tolni a m\u00e9hnyakr\u00e1ksejtek prolifer\u00e1ci\u00f3j\u00e1t, elnyomni a sejtmigr\u00e1ci\u00f3t, sejtciklus-meg\u00e1ll\u00e1st \u00e9s apopt\u00f3zist induk\u00e1lni. A kutat\u00e1s referenciaalapot ny\u00fajt a chamagu n\u00f6v\u00e9nyi er\u0151forr\u00e1sok tov\u00e1bbi fejleszt\u00e9s\u00e9hez \u00e9s hasznos\u00edt\u00e1s\u00e1hoz. A kutat\u00e1si eredm\u00e9nyeket a Natural Product Research and Development 2024. \u00e9vi 6. sz\u00e1m\u00e1ban tett\u00e9k k\u00f6zz\u00e9.<\/p>","protected":false},"excerpt":{"rendered":"

Enzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate In recent years, the incidence rate of cancer is increasing rapidly in China, and the prevention and treatment of cancer is urgent. Research shows that increasing the intake of cruciferous vegetables can effectively reduce the incidence rate of cancer. Brassicaceae vegetables contain abundant glucosinolates, also […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"yoast_head":"\nEnzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/chamagu-isothiocyanate\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Enzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Enzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate In recent years, the incidence rate of cancer is increasing rapidly in China, and the prevention and treatment of cancer is urgent. Research shows that increasing the intake of cruciferous vegetables can effectively reduce the incidence rate of cancer. 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