{"id":7458,"date":"2024-08-06T19:58:27","date_gmt":"2024-08-06T19:58:27","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7458"},"modified":"2024-08-06T19:58:27","modified_gmt":"2024-08-06T19:58:27","slug":"jinlu","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/fr\/jinlu\/","title":{"rendered":"Extraction, enrichissement et \u00e9tude de l'activit\u00e9 d'un inhibiteur de l'alpha-glucosidase du prunier Jinlu"},"content":{"rendered":"

Extraction, enrichissement et \u00e9tude de l'activit\u00e9 d'un inhibiteur de l'alpha-glucosidase du prunier Jinlu
\nL'\u03b1-glucosidase (AG) est une enzyme \u00e9troitement li\u00e9e au diab\u00e8te. Elle peut m\u00e9taboliser les hydrates de carbone en glucose, fructose et galactose qui peuvent \u00eatre absorb\u00e9s et utilis\u00e9s par le corps humain. L'inhibiteur de l'\u03b1-glucosidase (AGI) peut bloquer la combinaison de l'AG et du sucre et r\u00e9duire la glyc\u00e9mie \u00e0 jeun en inhibant de mani\u00e8re comp\u00e9titive l'activit\u00e9 de l'AG. Ces derni\u00e8res ann\u00e9es, avec l'augmentation du nombre de patients atteints de diab\u00e8te, les ingr\u00e9dients efficaces des m\u00e9dicaments chinois et tib\u00e9tains ont progressivement augment\u00e9 dans l'application clinique du traitement du diab\u00e8te. Les inhibiteurs naturels de l'\u03b1-glucosidase pr\u00e9sentent les avantages d'une efficacit\u00e9 stable, de la s\u00e9curit\u00e9, de faibles effets secondaires, etc.
\nPotentilla fruticosa L. est une plante arbustive \u00e0 feuilles caduques appartenant \u00e0 la famille des Rosaceae et au genre Potentilla, principalement r\u00e9partie dans les r\u00e9gions de haute altitude du plateau tib\u00e9tain de Qinghai. Les racines, les tiges, les feuilles, les fleurs et les branches du Jin Lu Mei ont une forte activit\u00e9 pharmacologique. Les feuilles sont couramment utilis\u00e9es comme additifs dans l'alimentation et les cosm\u00e9tiques, et les extraits de racines et de tiges ont de fortes propri\u00e9t\u00e9s de r\u00e9gulation immunitaire, avec un effet plus important que celui du Ci Wu Jia ; l'extrait de la branche est comestible, et des exp\u00e9riences in vitro ont montr\u00e9 qu'il a une bonne activit\u00e9 dans la r\u00e9duction des niveaux de cholest\u00e9rol et de glucose dans le sang. Des \u00e9tudes ant\u00e9rieures men\u00e9es par notre groupe de recherche sur la composition chimique des Primulaceae ont montr\u00e9 que le compos\u00e9 flavono\u00efde querc\u00e9tine-7-O - \u03b2 - D-glucuronide a \u00e9t\u00e9 isol\u00e9 pour la premi\u00e8re fois dans les Primulaceae et qu'il a une activit\u00e9 d'inhibiteur de l'alpha glucosidase.
\nLes m\u00e9thodes de s\u00e9paration et de purification des flavono\u00efdes v\u00e9g\u00e9taux comprennent principalement l'adsorption sur r\u00e9sine, la chromatographie liquide \u00e0 haute performance, etc. La m\u00e9thode d'adsorption sur r\u00e9sine est largement utilis\u00e9e pour l'enrichissement des flavono\u00efdes en raison de ses avantages : grande surface, grande capacit\u00e9 d'adsorption, bonne s\u00e9lectivit\u00e9, longue dur\u00e9e de vie de la r\u00e9sine et faible co\u00fbt d'exploitation. Cette \u00e9tude a utilis\u00e9 la m\u00e9thode de r\u00e9sine d'adsorption macroporeuse (AB-8) pour enrichir et inhiber l'activit\u00e9 AG du compos\u00e9 querc\u00e9tine-7-O - \u03b2 - D-glucuronide dans Prunus mume, fournissant une base th\u00e9orique pour le d\u00e9veloppement et l'utilisation complets des inhibiteurs naturels de l'\u03b1-glucosidase dans Prunus mume.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"
\nCet article prend le m\u00e9dicament tib\u00e9tain Jin Lu Mei comme objet de recherche et utilise la chromatographie liquide haute performance semi-pr\u00e9parative DAC-50 et la chromatographie liquide haute performance pour isoler pour la premi\u00e8re fois le compos\u00e9 hypoglyc\u00e9mique cible avec une grande puret\u00e9 \u00e0 partir du Jin Lu Mei. Une m\u00e9thode de s\u00e9paration des compos\u00e9s monom\u00e8res Quercetin-7-O - \u03b2 - D-glucuronide du Jin Lu Mei est \u00e9tablie. Les composants chimiques pr\u00e9sents dans le Jinlu Mei ont \u00e9t\u00e9 extraits \u00e0 l'aide de l'\u00e9thanol 75%. La solution d'extraction a \u00e9t\u00e9 s\u00e9par\u00e9e et segment\u00e9e en utilisant la chromatographie sur colonne AB-8 avec 0, 10%, 20%, 30%, 40%, et 50% solutions d'eau et d'\u00e9thanol. Les compos\u00e9s ont \u00e9t\u00e9 class\u00e9s pr\u00e9liminairement en fonction de leur polarit\u00e9, et les compos\u00e9s monom\u00e8res dans l'\u00e9luant AB-8 20% de Jinlumei ont \u00e9t\u00e9 principalement isol\u00e9s et pr\u00e9par\u00e9s.
\nL'analyse structurelle du compos\u00e9 a r\u00e9v\u00e9l\u00e9 qu'il s'agissait d'un compos\u00e9 flavono\u00efde. Les r\u00e9sultats de la d\u00e9tection de l'activit\u00e9 et de l'identification du type d'inhibiteur de ce compos\u00e9 ont montr\u00e9 que la querc\u00e9tine-7-O - \u03b2 - D-glucuronide \u00e9tait un inhibiteur comp\u00e9titif, avec le taux d'inhibition le plus \u00e9lev\u00e9 de 94,67%. Cela indique que le compos\u00e9 a une forte comp\u00e9titivit\u00e9 pour se lier \u00e0 l'AG, r\u00e9duisant consid\u00e9rablement la liaison de l'AG aux sucres, ce qui est coh\u00e9rent avec les r\u00e9sultats de recherches ant\u00e9rieures. Le mod\u00e8le de liaison possible entre le compos\u00e9 et l'AG a \u00e9t\u00e9 \u00e9tudi\u00e9 \u00e0 l'aide d'une m\u00e9thode d'ancrage mol\u00e9culaire assist\u00e9e par ordinateur, et le m\u00e9canisme d'action possible a \u00e9t\u00e9 explor\u00e9. Les trois anneaux benz\u00e9niques du noyau parent flavono\u00efde du compos\u00e9 ont form\u00e9 un \u00e9tat de liaison stable avec les r\u00e9sidus prot\u00e9iques AG par le biais de liaisons conjugu\u00e9es \u03c0 - \u03c0, de liaisons \u03c0 - alkyle, de liaisons conjugu\u00e9es \u03c0 - \u03c3, de liaisons hydrog\u00e8ne, etc. Ce compos\u00e9 a une bonne activit\u00e9 inhibitrice de l'\u03b1-glucosidase, ce qui indique que Jinlumei a le potentiel de d\u00e9velopper des m\u00e9dicaments pour traiter le diab\u00e8te, et fournit une base th\u00e9orique scientifique pour la recherche et le d\u00e9veloppement d'ingr\u00e9dients pharmaceutiques hypoglyc\u00e9miants de Jinlumei.<\/p>","protected":false},"excerpt":{"rendered":"

Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum \u03b1 – glucosidase (AG) is an enzyme closely related to diabetes. It can metabolize carbohydrates into glucose, fructose and galactose that can be absorbed and utilized by the human body in the body. \u03b1 – glucosidase inhibitor (AGI) can block the combination […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nExtraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/fr\/jinlu\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum \u03b1 – glucosidase (AG) is an enzyme closely related to diabetes. It can metabolize carbohydrates into glucose, fructose and galactose that can be absorbed and utilized by the human body in the body. \u03b1 – glucosidase inhibitor (AGI) can block the combination […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangextracts.com\/fr\/jinlu\/\" \/>\n<meta property=\"og:site_name\" content=\"China Chemical Manufacturer\" \/>\n<meta property=\"article:published_time\" content=\"2024-08-06T19:58:27+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg\" \/>\n<meta name=\"author\" content=\"longcha9\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u00c9crit par\" \/>\n\t<meta name=\"twitter:data1\" content=\"longcha9\" \/>\n\t<meta name=\"twitter:label2\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangextracts.com\/jinlu\/\",\"url\":\"https:\/\/longchangextracts.com\/jinlu\/\",\"name\":\"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum - China Chemical Manufacturer\",\"isPartOf\":{\"@id\":\"https:\/\/longchangextracts.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/longchangextracts.com\/jinlu\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/longchangextracts.com\/jinlu\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg\",\"datePublished\":\"2024-08-06T19:58:27+00:00\",\"dateModified\":\"2024-08-06T19:58:27+00:00\",\"author\":{\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869\"},\"breadcrumb\":{\"@id\":\"https:\/\/longchangextracts.com\/jinlu\/#breadcrumb\"},\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/longchangextracts.com\/jinlu\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-FR\",\"@id\":\"https:\/\/longchangextracts.com\/jinlu\/#primaryimage\",\"url\":\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg\",\"contentUrl\":\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/longchangextracts.com\/jinlu\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/longchangextracts.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/longchangextracts.com\/#website\",\"url\":\"https:\/\/longchangextracts.com\/\",\"name\":\"China Chemical Manufacturer\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/longchangextracts.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"fr-FR\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869\",\"name\":\"longcha9\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-FR\",\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g\",\"caption\":\"longcha9\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Extraction, enrichissement et \u00e9tude de l'activit\u00e9 d'un inhibiteur de l'alpha-glucosidase de la prune de Jinlu - China Chemical Manufacturer","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/longchangextracts.com\/fr\/jinlu\/","og_locale":"fr_FR","og_type":"article","og_title":"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum - China Chemical Manufacturer","og_description":"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum \u03b1 – glucosidase (AG) is an enzyme closely related to diabetes. It can metabolize carbohydrates into glucose, fructose and galactose that can be absorbed and utilized by the human body in the body. \u03b1 – glucosidase inhibitor (AGI) can block the combination […]","og_url":"https:\/\/longchangextracts.com\/fr\/jinlu\/","og_site_name":"China Chemical Manufacturer","article_published_time":"2024-08-06T19:58:27+00:00","og_image":[{"url":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg"}],"author":"longcha9","twitter_card":"summary_large_image","twitter_misc":{"\u00c9crit par":"longcha9","Dur\u00e9e de lecture estim\u00e9e":"5 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/longchangextracts.com\/jinlu\/","url":"https:\/\/longchangextracts.com\/jinlu\/","name":"Extraction, enrichissement et \u00e9tude de l'activit\u00e9 d'un inhibiteur de l'alpha-glucosidase de la prune de Jinlu - China Chemical Manufacturer","isPartOf":{"@id":"https:\/\/longchangextracts.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/longchangextracts.com\/jinlu\/#primaryimage"},"image":{"@id":"https:\/\/longchangextracts.com\/jinlu\/#primaryimage"},"thumbnailUrl":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg","datePublished":"2024-08-06T19:58:27+00:00","dateModified":"2024-08-06T19:58:27+00:00","author":{"@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869"},"breadcrumb":{"@id":"https:\/\/longchangextracts.com\/jinlu\/#breadcrumb"},"inLanguage":"fr-FR","potentialAction":[{"@type":"ReadAction","target":["https:\/\/longchangextracts.com\/jinlu\/"]}]},{"@type":"ImageObject","inLanguage":"fr-FR","@id":"https:\/\/longchangextracts.com\/jinlu\/#primaryimage","url":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg","contentUrl":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-36.jpg"},{"@type":"BreadcrumbList","@id":"https:\/\/longchangextracts.com\/jinlu\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/longchangextracts.com\/"},{"@type":"ListItem","position":2,"name":"Extraction, Enrichment, and Activity Study of an Alpha Glucosidase Inhibitor from Jinlu Plum"}]},{"@type":"WebSite","@id":"https:\/\/longchangextracts.com\/#website","url":"https:\/\/longchangextracts.com\/","name":"Fabricant chinois de produits chimiques","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/longchangextracts.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"fr-FR"},{"@type":"Person","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869","name":"longcha9","image":{"@type":"ImageObject","inLanguage":"fr-FR","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","caption":"longcha9"}}]}},"_links":{"self":[{"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/posts\/7458"}],"collection":[{"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/comments?post=7458"}],"version-history":[{"count":1,"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/posts\/7458\/revisions"}],"predecessor-version":[{"id":7467,"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/posts\/7458\/revisions\/7467"}],"wp:attachment":[{"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/media?parent=7458"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/categories?post=7458"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longchangextracts.com\/fr\/wp-json\/wp\/v2\/tags?post=7458"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}