{"id":5306,"date":"2024-08-04T18:18:38","date_gmt":"2024-08-04T18:18:38","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5306"},"modified":"2024-08-04T18:18:38","modified_gmt":"2024-08-04T18:18:38","slug":"green-calyx-plum","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/fr\/calice-vert-prune\/","title":{"rendered":"Caract\u00e9risation des diff\u00e9rences de composition et de l'activit\u00e9 antioxydante de la prune Green Calyx \u00e0 diff\u00e9rents stades de d\u00e9veloppement par UPLC-QE-MS combin\u00e9e \u00e0 l'UPLC"},"content":{"rendered":"

Caract\u00e9risation des diff\u00e9rences de composition et de l'activit\u00e9 antioxydante de la prune Green Calyx \u00e0 diff\u00e9rents stades de d\u00e9veloppement par UPLC-QE-MS combin\u00e9e \u00e0 l'UPLC
\nLe prunier \u00e0 s\u00e9pales verts est un bourgeon floral s\u00e9ch\u00e9 de Prunus mume (Sieb.) Sieb. et Zucc, une plante de la famille des Rosac\u00e9es. Il figure dans l'\u00e9dition 2020 de la pharmacop\u00e9e chinoise en tant que m\u00e9dicament traditionnel chinois couramment utilis\u00e9. Elle a pour effet d'apaiser le foie, de favoriser la digestion, de r\u00e9soudre les mucosit\u00e9s et de disperser les nodules. Il est couramment utilis\u00e9 pour traiter des maladies telles que les douleurs du foie et du qi de l'estomac, la d\u00e9pression et la scrofule. Les documents anciens et la recherche moderne sugg\u00e8rent que les fleurs de prunier, avec leurs \"s\u00e9pales verts\", sont excellentes pour un usage m\u00e9dicinal. Elles se caract\u00e9risent par un s\u00e9pale vert gris\u00e2tre et des p\u00e9tales jaunes et blancs, avec un ar\u00f4me parfum\u00e9 et une bonne efficacit\u00e9 clinique. En tant qu'herbe m\u00e9dicinale et comestible, le prunier \u00e0 calice vert peut \u00e9galement \u00eatre utilis\u00e9 pour pr\u00e9parer du th\u00e9, des p\u00e2tisseries et d'autres aliments. Elle fleurit en hiver, fra\u00eeche et \u00e9l\u00e9gante, et figure sur la liste des fleurs \u00e0 haute valeur ornementale. L'application globale du prunier vert fait l'objet d'une attention croissante.
\nDes chercheurs nationaux et \u00e9trangers ont men\u00e9 des recherches approfondies sur la composition chimique et les effets pharmacologiques des fleurs de prunier. Les composants ph\u00e9nylpropano\u00efdes et flavono\u00efdes, repr\u00e9sent\u00e9s par l'acide chlorog\u00e9nique, constituent la principale base de l'activit\u00e9 pharmacologique de la fleur de prunier. Leurs effets antioxydants, antiagr\u00e9gants plaquettaires, anti-m\u00e9lanine et antid\u00e9presseurs ont \u00e9t\u00e9 largement rapport\u00e9s. Ces derni\u00e8res ann\u00e9es, les flavono\u00efdes pr\u00e9sents dans les fleurs de prunier ont fait l'objet d'une attention croissante en raison de leurs propri\u00e9t\u00e9s antioxydantes et antid\u00e9pressives. L'acide chlorog\u00e9nique, l'isorhamn\u00e9tine, la querc\u00e9tine, l'isoquercitrine, l'hyperoside, la rutine et d'autres flavono\u00efdes ont \u00e9t\u00e9 isol\u00e9s et identifi\u00e9s dans les fleurs de prunier. Cependant, il existe peu de rapports sur la composition chimique globale du prunier \u00e0 calice vert, ce qui entrave la recherche et le d\u00e9veloppement approfondis de ses m\u00e9tabolites secondaires.
\nLes m\u00e9tabolites secondaires des plantes m\u00e9dicinales ont diverses activit\u00e9s biologiques et constituent une base mat\u00e9rielle importante permettant aux plantes m\u00e9dicinales d'exercer leurs effets pharmacologiques. Les recherches ont montr\u00e9 que les diff\u00e9rents stades de croissance et de d\u00e9veloppement des plantes sont des facteurs importants qui influencent l'accumulation des m\u00e9tabolites secondaires dans les plantes. Pour les plantes m\u00e9dicinales florales en particulier, la p\u00e9riode de r\u00e9colte peut affecter de mani\u00e8re significative l'accumulation de m\u00e9tabolites secondaires. Pour \u00e9tudier les changements dynamiques des composants chimiques et les diff\u00e9rences d'activit\u00e9 antioxydante dans les organes du prunier vert \u00e0 diff\u00e9rents stades de d\u00e9veloppement, on a d'abord utilis\u00e9 la chromatographie liquide \u00e0 ultra-haute performance, la spectrom\u00e9trie de masse \u00e0 trappe orbitale \u00e0 champ \u00e9lectrostatique quadripolaire (UPLC-QE-MS) pour l'analyse qualitative afin de clarifier la composition chimique du prunier vert ; on a ensuite utilis\u00e9 la spectrophotom\u00e9trie UV ? La spectrophotom\u00e9trie visible a \u00e9t\u00e9 utilis\u00e9e pour d\u00e9terminer la teneur totale en flavono\u00efdes et en ph\u00e9nylpropano\u00efdes de 18 lots d'\u00e9chantillons de prunes vertes \u00e0 trois stades de d\u00e9veloppement ; En outre, la chromatographie liquide \u00e0 ultra-haute performance (UPLC) a \u00e9t\u00e9 utilis\u00e9e pour d\u00e9terminer la teneur de huit composants indicateurs (acide n\u00e9ochlorog\u00e9nique, acide chlorog\u00e9nique, acide caf\u00e9ique, rutine, hyperoside, isoquercitrine, acide isochlorog\u00e9nique B et quercitrine) dans des \u00e9chantillons de prunes vertes, et les diff\u00e9rences dans la teneur des huit composants \u00e0 diff\u00e9rents stades de d\u00e9veloppement ont \u00e9t\u00e9 compar\u00e9es ; La m\u00e9thode DPPH a \u00e9t\u00e9 utilis\u00e9e pour d\u00e9terminer l'activit\u00e9 antioxydante, et une analyse de corr\u00e9lation a \u00e9t\u00e9 r\u00e9alis\u00e9e entre la teneur en composants totaux et en composants indicateurs et l'activit\u00e9 antioxydante. Les r\u00e9sultats de la recherche fournissent une base scientifique pour clarifier les diff\u00e9rences de composition chimique et d'activit\u00e9 du prunier vert \u00e0 diff\u00e9rents stades de d\u00e9veloppement, et pour d\u00e9terminer la p\u00e9riode de r\u00e9colte optimale des mat\u00e9riaux m\u00e9dicinaux du prunier vert.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"<\/p>\n

Dans cette exp\u00e9rience, un total de 328 m\u00e9tabolites secondaires a \u00e9t\u00e9 d\u00e9tect\u00e9 dans le calice vert de la prune en utilisant la m\u00e9thode UPLC-QE-MS. Les flavono\u00efdes et les ph\u00e9nylpropano\u00efdes repr\u00e9sentaient une proportion \u00e9lev\u00e9e, avec respectivement 67 et 50 compos\u00e9s. Parmi les flavono\u00efdes, les flavonols repr\u00e9sentaient la proportion la plus \u00e9lev\u00e9e, et des composants tels que l'hyperoside et l'isoquercitrine, qui servent de normes de qualit\u00e9 pour les fleurs de prunier, appartiennent tous \u00e0 cette classe de substances. Des \u00e9tudes ont montr\u00e9 que les flavonols jouent un r\u00f4le r\u00e9gulateur important dans le d\u00e9veloppement des organes floraux. Un seul composant des anthocyanes a \u00e9t\u00e9 d\u00e9tect\u00e9, ce qui peut \u00eatre li\u00e9 \u00e0 la couleur blanche de la fleur de prunier \u00e0 calice vert.<\/p>\n

Une comparaison plus pouss\u00e9e des changements dans la composition du calice vert \u00e0 diff\u00e9rents stades de d\u00e9veloppement a montr\u00e9 que la teneur en flavono\u00efdes totaux et en ph\u00e9nylpropano\u00efdes totaux \u00e9tait la plus \u00e9lev\u00e9e pendant le stade du bourgeon, et qu'elle diminuait progressivement avec le degr\u00e9 d'ouverture de la fleur. Les r\u00e9sultats de la d\u00e9termination de la teneur de 8 composants indicateurs (acide n\u00e9ochlorog\u00e9nique, acide chlorog\u00e9nique, acide caf\u00e9ique, rutine, hyperoside, isoquercitrine, acide isochlorog\u00e9nique B, quercitrine) ont montr\u00e9 des diff\u00e9rences significatives entre le stade du bourgeon et le stade de l'ouverture compl\u00e8te, l'acide n\u00e9ochlorog\u00e9nique, l'acide chlorog\u00e9nique, la rutine, l'isoquercitrine et la quercitrine montrant des diff\u00e9rences extr\u00eamement significatives (P<0.0001) ; l'analyse de la st\u0153chiom\u00e9trie chimique a montr\u00e9 qu'il y avait certaines diff\u00e9rences dans la composition chimique de la prune verte du calice au cours des trois p\u00e9riodes, et que l'acide chlorog\u00e9nique pouvait \u00eatre utilis\u00e9 comme marqueur de qualit\u00e9 diff\u00e9rentielle. Dans l'ensemble, le prunier vert au stade du bourgeon pr\u00e9sente des avantages en termes de composition chimique et d'activit\u00e9 antioxydante, avec des diff\u00e9rences significatives par rapport aux stades semi-ouvert et compl\u00e8tement ouvert. Par cons\u00e9quent, la p\u00e9riode de r\u00e9colte des mati\u00e8res m\u00e9dicinales devrait \u00eatre contr\u00f4l\u00e9e autant que possible pendant le stade du bourgeon.
\nEn r\u00e9sum\u00e9, cette exp\u00e9rience a utilis\u00e9 la m\u00e9thode UPLC-QE-MS combin\u00e9e \u00e0 la m\u00e9thode UPLC pour analyser qualitativement et quantitativement la composition chimique et les diff\u00e9rences de la prune verte \u00e0 diff\u00e9rents stades de d\u00e9veloppement, et a compar\u00e9 ses diff\u00e9rences d'activit\u00e9 antioxydante par la m\u00e9thode DPPH. Cela fournit une base scientifique pour les changements dynamiques dans la composition chimique du prunier vert et la p\u00e9riode optimale de r\u00e9colte des mat\u00e9riaux m\u00e9dicinaux.<\/p>","protected":false},"excerpt":{"rendered":"

Characterization of Composition Differences and Antioxidant Activity of Green Calyx Plum at Different Development Stages by UPLC-QE-MS Combined with UPLC Green sepal plum is a dried flower bud of Prunus mume (Sieb.) Sieb. et Zucc., a plant in the Rosaceae family. It is included in the 2020 edition of the Chinese Pharmacopoeia as a commonly […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nCharacterization of Composition Differences and Antioxidant Activity of Green Calyx Plum at Different Development Stages by UPLC-QE-MS Combined with UPLC - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/fr\/calice-vert-prune\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Characterization of Composition Differences and Antioxidant Activity of Green Calyx Plum at Different Development Stages by UPLC-QE-MS Combined with UPLC - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Characterization of Composition Differences and Antioxidant Activity of Green Calyx Plum at Different Development Stages by UPLC-QE-MS Combined with UPLC Green sepal plum is a dried flower bud of Prunus mume (Sieb.) 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