{"id":5203,"date":"2024-08-04T17:48:42","date_gmt":"2024-08-04T17:48:42","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5203"},"modified":"2024-08-04T17:48:42","modified_gmt":"2024-08-04T17:48:42","slug":"mushroom","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/fr\/mushroom\/","title":{"rendered":"S\u00e9paration et purification efficaces d'une nouvelle enzyme fibrinolytique \u00e0 partir du corps de fructification du champignon \u00e0 bois de velours"},"content":{"rendered":"

S\u00e9paration et purification efficaces d'une nouvelle enzyme fibrinolytique \u00e0 partir du corps de fructification du champignon \u00e0 bois de velours
\nLes maladies thromboemboliques ont un taux d'invalidit\u00e9 et de mortalit\u00e9 \u00e9lev\u00e9. La fibrine \u00e9tant le principal composant des caillots sanguins, la promotion de la d\u00e9gradation de la fibrine est un moyen courant de pr\u00e9venir et de traiter les maladies thrombotiques. Les enzymes fibrinolytiques exog\u00e8nes, telles que l'urokinase (UK) et la streptokinase (SK), sont largement utilis\u00e9es dans le traitement clinique des maladies thromboemboliques en raison de leur capacit\u00e9 \u00e0 hydrolyser indirectement ou directement la fibrine. Bien qu'ils aient une efficacit\u00e9 thrombolytique significative, ils pr\u00e9sentent \u00e9galement certaines limites d'application telles qu'une demi-vie courte et des effets secondaires importants. Par cons\u00e9quent, la recherche de nouvelles enzymes fibrinolytiques pr\u00e9sentant une forte sp\u00e9cificit\u00e9, une bonne s\u00e9curit\u00e9 et peu d'effets secondaires est devenue un point chaud de la recherche actuelle.<\/p>\n

Les champignons comestibles sont riches en prot\u00e9ines, polysaccharides, terp\u00e8nes, acides amin\u00e9s, st\u00e9rols, nucl\u00e9osides et autres substances, qui ont diverses fonctions physiologiques telles que l'antioxydation, la lutte contre les tumeurs, la r\u00e9gulation immunitaire et la r\u00e9gulation neuronale. \u00c0 l'heure actuelle, la recherche sur les enzymes fibrinolytiques d\u00e9riv\u00e9es de champignons comestibles et leurs propri\u00e9t\u00e9s fonctionnelles fait l'objet d'une grande attention. Des enzymes fibrinolytiques ont \u00e9t\u00e9 isol\u00e9es et extraites de divers champignons comestibles, tels que Lentinus edodes, Cordyceps Militaris, Agrocybe aegerita, Tricholoma sejunctum, Lepista nuda, Pleurotus ferulae, etc.<\/p>\n

Le champignon bois de cerf est un champignon comestible et m\u00e9dicinal qui se d\u00e9compose dans le bois et qui est largement r\u00e9pandu dans les r\u00e9gions temp\u00e9r\u00e9es de l'h\u00e9misph\u00e8re nord. Il a des fonctions physiologiques telles que l'antioxydation, le renforcement de l'immunit\u00e9, la suppression des tumeurs et la r\u00e9duction des lipides sanguins. Les exp\u00e9riences pr\u00e9liminaires ont montr\u00e9 que l'extrait du corps fructif\u00e8re du champignon bois de velours contenait une plasmine \u00e0 haute activit\u00e9, qui \u00e9tait significativement plus \u00e9lev\u00e9e que la plasmine rapport\u00e9e \u00e0 partir d'autres sources de champignons comestibles. Cet article pr\u00e9sente la m\u00e9thode de s\u00e9paration et de purification de l'enzyme fibrinolytique contenue dans le corps fructif\u00e8re du champignon \u00e0 bois de velours, et identifie de mani\u00e8re pr\u00e9liminaire l'enzyme. \u00c0 l'heure actuelle, notre groupe de recherche n'a pas vu d'autres rapports relatifs \u00e0 l'enzyme fibrinolytique dans le champignon \u00e0 bois de velours. Cette \u00e9tude peut servir de base \u00e0 la r\u00e9alisation de tests fonctionnels thrombolytiques et antithrombotiques de l'enzyme fibrinolytique du champignon \u00e0 bois de velours et jeter les bases th\u00e9oriques de son utilisation dans le d\u00e9veloppement d'aliments fonctionnels.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"Dans cette \u00e9tude, une enzyme fibrinolytique a \u00e9t\u00e9 isol\u00e9e et purifi\u00e9e \u00e0 partir du corps de fructification du champignon \u00e0 bois de velours en utilisant une technologie moderne de s\u00e9paration chromatographique. La s\u00e9quence N-terminale de douze acides amin\u00e9s de l'enzyme fibrinolytique est Gly Ala Val Thr Gln Asn Ala Pro Trp Gly Leu. Apr\u00e8s comparaison avec la base de donn\u00e9es NCBI, l'enzyme fibrinolytique a \u00e9t\u00e9 identifi\u00e9e comme une nouvelle enzyme fibrinolytique. L'activit\u00e9 sp\u00e9cifique de l'enzyme fibrinolytique est de 4105,78U\/mg, le facteur de purification est de 206,09 et le taux de r\u00e9cup\u00e9ration de l'activit\u00e9 est de 30,91%. L'\u00e9lectrophor\u00e8se Native PAGE et l'\u00e9lectrophor\u00e8se SDS-PAGE ont r\u00e9v\u00e9l\u00e9 que le poids mol\u00e9culaire relatif de l'enzyme fibrinolytique \u00e9tait de 30,9 kDa. Les r\u00e9sultats ci-dessus indiquent que la nouvelle enzyme fibrinolytique d\u00e9riv\u00e9e du corps de fructification des champignons \u00e0 bois de velours poss\u00e8de un bon potentiel thrombolytique et peut \u00eatre utilis\u00e9e pour le d\u00e9veloppement d'aliments fonctionnels destin\u00e9s \u00e0 r\u00e9duire la tendance \u00e0 la thrombophilie.<\/p>","protected":false},"excerpt":{"rendered":"

Efficient separation and purification of novel fibrinolytic enzyme from the fruiting body of velvet antler mushroom Thromboembolic diseases have a high disability and mortality rate. Fibrin is the main component of blood clots, therefore, promoting fibrin degradation is a common means of preventing and treating thrombotic diseases. Exogenous fibrinolytic enzymes, such as urokinase (UK) and […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nEfficient separation and purification of novel fibrinolytic enzyme from the fruiting body of velvet antler mushroom - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/fr\/mushroom\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Efficient separation and purification of novel fibrinolytic enzyme from the fruiting body of velvet antler mushroom - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Efficient separation and purification of novel fibrinolytic enzyme from the fruiting body of velvet antler mushroom Thromboembolic diseases have a high disability and mortality rate. Fibrin is the main component of blood clots, therefore, promoting fibrin degradation is a common means of preventing and treating thrombotic diseases. 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