Study on secondary metabolites of endophytic fungus Fusarium guttiforme in plants
Endophytic fungi refer to a group of microorganisms that live in healthy plant tissues but do not cause plant infections and diseases. They have unique biological characteristics, interact and benefit with host plants, and produce various important secondary metabolites. They can be used in pharmaceuticals, agriculture, and the food industry with a range of biological activities.
Fusarium is a type of filamentous fungus widely distributed in soil and plant bodies. In recent years, many scholars have studied the secondary metabolites of Fusarium fungi and found that they can not only produce three main fungal toxins: fumonis, trichothecenes, and zearalenones, but also produce terpenes, amides, quinones, and polyketones with significant biological activities. Through research on relevant literature, it was found that there is relatively little research on the secondary metabolites of the endophytic fungus Fusarium gutiforme in plants. In order to further enrich the chemical composition of Fusarium gutiforme metabolites and consider the impact of solid-state fermentation on their secondary metabolites, this study used various chromatographic separation methods and modern spectroscopic techniques to investigate the chemical composition of the secondary metabolites of rice fermentation by the endophytic fungus Fusarium gutiforme. At the same time, the obtained compounds were screened for in vitro anti-inflammatory activity, aiming to discover novel and active lead molecules and enrich the secondary metabolites of the genus Fusarium.

 

This study investigated the endophytic bacteria F. in plants Seven compounds were isolated and identified from the ethyl acetate fraction of rice fermented by Gutiforme. Among them, compound 1 is a novel biphenyl derivative containing peroxide bonds, while compounds 2-7 are benzene derivatives, phenolic acids, steroids, ergosterol, and furfural compounds, all of which were isolated from this fungus for the first time, greatly enriching the structural diversity of secondary metabolites in the genus Fusarium. At the same time, most dimeric or polymeric derivatives have strong pharmacological activity and stability, with more research on dimeric derivatives. Paik et al. reported a series of dimeric derivatives containing benzene ring structures, among which phthalate ester dimers have good selectivity and cytotoxicity against human cervical cancer, and have no cytotoxic effect on normal cervical cells. Artemisinin dimers linked by alkyl, ester, ether bonds, etc. often have higher in vitro and in vivo anti malaria activity, lower toxic side effects, and stable physicochemical properties than mononuclear compounds. In addition, previous reports have shown that derivatives of benzene rings, phenols, and steroids have anti-inflammatory, antioxidant, anti-tumor, antibacterial, and other activities. Therefore, this article discusses F The anti-inflammatory activities of compounds 1-7 isolated from Gutiforme were preliminarily evaluated in vitro. The results showed that compounds 1 and 2 exhibited certain NO production inhibition activity, with IC50 values of 48.1 and 46.6 μ mol/L, which preliminarily elucidated the F The active substance basis of guttiforme provides reference for subsequent pharmacological experiments and can also provide certain scientific value for research related to Fusarium.