{"id":7523,"date":"2024-08-06T20:19:48","date_gmt":"2024-08-06T20:19:48","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7523"},"modified":"2024-08-06T20:19:48","modified_gmt":"2024-08-06T20:19:48","slug":"sophora-alopecuroides","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/da\/sophora-alopecuroides\/","title":{"rendered":"Unders\u00f8gelse af den antiinflammatoriske effekt og mekanisme af Sophora alopecuroides p\u00e5 lipopolysakkarid-induceret inflammation i RAW264.7-celler"},"content":{"rendered":"

Unders\u00f8gelse af den antiinflammatoriske effekt og mekanisme af Sophora alopecuroides p\u00e5 lipopolysakkarid-induceret inflammation i RAW264.7-celler
\nInflammation er en forsvarsreaktion, der opst\u00e5r, n\u00e5r kroppen angribes eller inficeres af skadelige indre og ydre stimuli som f.eks. patogener. Det udl\u00f8ser en immunreaktion for at beskytte kroppen. N\u00e5r immunsystemet aktiveres overdrevent eller kontinuerligt, kan det f\u00f8re til en r\u00e6kke inflammationsrelaterede sygdomme som f.eks. leddegigt, inflammatorisk tarmsygdom, tumorer osv. og i sidste ende resultere i permanent eller midlertidig inflammation. Makrofager spiller en central rolle i initiering, vedligeholdelse og lindring af inflammatoriske reaktioner, og deres vedvarende ophobning og aktivering er tegn p\u00e5 kronisk inflammation. Lipopolysaccharid (LPS) kan aktivere makrofager og fremme deres udskillelse af inflammatoriske mediatorer s\u00e5som tumornekrosefaktor alfa (TNF-\u03b1), interleukin-1 \u03b2 (IL-1 \u03b2), interleukin-6 (IL-6), nitrogenoxid (NO) osv. Den overdrevne frigivelse af disse inflammatoriske mediatorer kan f\u00f8re til organskader og kroniske sygdomme. NF - \u03ba B spiller en vigtig rolle i reguleringen af processer som celleapoptose og inflammatorisk respons og er et vigtigt m\u00e5l for antiinflammatorisk behandling.<\/p>\n

Sophora alopecuroides L, ogs\u00e5 kendt som Sophora alopecuroides, er en plante, der h\u00f8rer til Leguminosae-familien i Sophora-sl\u00e6gten. Den er udbredt i forskellige provinser i det nordvestlige Kina og i de centralasiatiske lande, og dens funktioner er at fjerne varme og afgifte, lindre smerter og bet\u00e6ndelse, udt\u00f8rre fugt og stoppe dysenteri. Det er en vigtig l\u00e6geplante i den nordvestlige del af Kina. Sophora alopecuroides indeholder hovedsageligt alkaloider, flavonoider, organiske syrer, polysaccharider, flygtige olier, steroider, fenoler og andre komponenter. Alkaloider har antiinflammatoriske, anticancer-, antibakterielle og immunregulerende virkninger, flavonoider har antiinflammatoriske, antivirale og antioxidante virkninger, og polysaccharider har immunregulerende og antitumorfunktioner, som effektivt kan forbedre kroppens immunitet og spille en vigtig rolle i antitumor- og immunforsvaret. Ovenst\u00e5ende unders\u00f8gelser er alle baseret p\u00e5 en bestemt kemisk sammens\u00e6tningsgruppe af Sophora alopecuroides, og traditionel kinesisk medicin har karakter af kombinerede effekter med flere komponenter, flere m\u00e5l og flere veje, som kan opn\u00e5 den samme eller endda bedre terapeutiske effekt med lavere l\u00e6gemiddeldoser, herunder effektiv delkompatibilitet og effektiv ingredienskompatibilitet. Derfor er det n\u00f8dvendigt at unders\u00f8ge, om forskellige kemiske sammens\u00e6tningsgrupper af Sophora alopecuroides kan forbedre effekten. Denne artikel bruger LPS-inducerede RAW 264.7-celler som en inflammatorisk model til screening af antiinflammatoriske l\u00e6gemidler i Sophora alopecuroides, og unders\u00f8ger det konventionelle vandafkogningsekstrakt (WDE), vandultralydsekstrakt (WUE), 75% ethanolrefluxekstrakt (ERE), 75% ethanol-ultralydsekstrakt (EUE) samt det samlede alkaloidekstrakt (TAE), det samlede flavonoidekstrakt (TFE), det samlede polysaccharidekstrakt (TPE) af Sophora alopecuroides og deres ni kombinationer af forskellige effektive dele. Den anti-inflammatoriske effekt og virkningsmekanisme af (1:1:1, 1:2:2, 1:3:3, 2:1:2, 2:2:3, 2:3:1, 3:1:3, 3:2:1, 3:3:2).<\/p>\n

\"\"\"\"\"\"\"\"\"\"
\nMakrofager deltager i kroppens immunrespons. N\u00e5r de stimuleres af LPS, udl\u00f8ser makrofager signalveje (s\u00e5som NF-\u03ba B) for at fremme produktionen af pro-inflammatoriske mediatorer s\u00e5som NO, TNF-\u03b1, IL-1 \u03b2, IL-6 osv. Overdreven produktion af NO er t\u00e6t forbundet med forekomsten af inflammation. En stigning i niveauerne af TNF - \u03b1 og IL-6 kan fremme cellernes udskillelse af flere proinflammatoriske cytokiner. IL-6 er et vigtigt proinflammatorisk cytokin, der kan fremkalde aktivering af B- og T-celler og deltage i kroppens immunrespons. IL-1 \u03b2 opreguleres ved akut eller kronisk inflammation. Resultaterne af denne unders\u00f8gelse viste, at efter LPS-behandling af RAW 264.7 steg frigivelsen af NO, TNF-\u03b1, IL-6 og IL-1 \u03b2 betydeligt, hvilket tyder p\u00e5 en vellykket etablering af en in vitro-bet\u00e6ndelsesmodel. Sophora alopecuroides WDE, WUE, EUE, ERE og deres TAE, TFE, TPE kan alle h\u00e6mme produktionen af NO, TNF - \u03b1, IL-6 og IL-1 \u03b2 i RAW 264.7-celler stimuleret af LPS, herunder TFE, EUE, ERE Den h\u00e6mmende virkning af TAE er mere signifikant Efter blanding af TFE og TPE i forskellige forhold blev det konstateret, at de kunne h\u00e6mme frigivelsen af TNF - \u03b1 i varierende grad. Med undtagelse af kombinationerne 1:1:1 og 1:1:2 havde de syv andre kombinationer en bedre h\u00e6mmende effekt p\u00e5 frigivelsen af TNF - \u03b1, end n\u00e5r de virkede alene. Kombinationen 2:1:2 havde den bedste h\u00e6mmende effekt, hvilket viser en betydelig synergistisk effekt.<\/p>\n

For at udforske den molekyl\u00e6re mekanisme i Sophora alopecuroides' antiinflammatoriske aktivitet brugte denne unders\u00f8gelse WB til at p\u00e5vise udtrykket af n\u00f8gleproteinerne IkB \u03b1, NF - \u03ba B p65 og p-IkB \u03b1, p-NF - \u03ba B p65 i NF - \u03ba B-signalvejen. NF - \u03ba B er en vigtig regulerende faktor i inflammation, som kan regulere transkriptionen af mange vigtige inflammatoriske mediatorer, s\u00e5som IL-1 \u03b2, IL-6, TNF - \u03b1 osv. LPS binder sig til TLR4-receptorer for at aktivere NF - \u03ba B-signalvejen, som er et vigtigt led i at f\u00e5 makrofager til at frigive inflammatoriske mediatorer. N\u00e5r cellerne stimuleres, bliver det h\u00e6mmende protein IkBa, der binder til NF - \u03ba B, hurtigt fosforyleret til p-IkB \u03b1 under p\u00e5virkning af IkB \u03b1-kinase, hvilket frigiver aktivt p-NF - \u03ba B p65 i kernen og derved fremmer syntesen og udskillelsen af inflammationsrelaterede faktorer. I de senere \u00e5r har der v\u00e6ret omfattende rapporter om de antiinflammatoriske virkninger af alkaloider, flavonoider og polysaccharider. For eksempel har unders\u00f8gelser vist, at Sophora flavescens-alkaloider kan d\u00e6mpe LPS-induceret inflammation i RAW 264.7-makrofager ved at h\u00e6mme IkB \u03b1-fosforylering og p-NF - \u03ba B p65-kernetranslokation. Zhao et al. fandt, at Sophora flavescens samlede alkaloider kan lindre dextransulfatinduceret colitis ved at h\u00e6mme NF - \u03ba B-aktivering. Unders\u00f8gelser har vist, at flavonoider i traditionel kinesisk medicin kan reducere fosforyleringsniveauerne af proteinerne IkB \u03b1 og p65 i NF - \u03ba B-signalvejen for at ud\u00f8ve antiinflammatoriske virkninger. Li et al. fandt, at Sophora flavescens samlede flavonoider kan ud\u00f8ve immunmodulerende virkninger p\u00e5 ulcerativ colitis ved at h\u00e6mme aktiveringen af JAK2\/STAT3-signalvejen, og Cistanche polysaccharider kan h\u00e6mme aktiveringen af JAK2\/STAT3-signalvejen. NF - \u03ba B MAPK og STAT3-aktivering bruges til at h\u00e6mme LPS-induceret inflammation i RAW 264.7-makrofager, mens Qu et al.s unders\u00f8gelse viste, at polysaccharider fra Pinus koraiensis kan h\u00e6mme LPS-induceret inflammation ved at regulere Nrf2\/HO-1-signalvejen. P\u00e5 nuv\u00e6rende tidspunkt er der relativt lidt forskning i effekten af kombinationen af alkaloider, flavonoider og polysaccharider p\u00e5 inflammation. Denne unders\u00f8gelse viste, at n\u00e5r kombinationen af TAE, TFE og TPE i Sophora alopecuroides blev brugt, blev fosforyleringen af NF - \u03ba B p65- og IkB \u03b1-proteiner reduceret betydeligt, og udtrykket af p-IkB \u03b1- og p-NF - \u03ba B p65-proteiner blev h\u00e6mmet. Dette indikerer, at kombinationen af Sophora alopecuroides samlede biomasse, samlede flavonoider og samlede polysaccharider kan lindre LPS-induceret inflammation i RAW 264.7-makrofager ved at h\u00e6mme aktiveringen af NF - \u03ba B-vejen.<\/p>\n

Sammenfattende sammenlignede denne unders\u00f8gelse hovedsageligt den antiinflammatoriske aktivitet af konventionelle WDE, WUE, EUE, ERE og deres forskellige effektive dele TAE, TFE, TPE og ni kombinationer (1:1:1, 1:2:2, 1:3:3, 2:1:2, 2:2:3, 2:3:1, 3:1:3, 3:2:1, 3:3:2) af Sophora alopecuroides in vitro. Det viste sig, at kombinationen af TAE, TFE, TPE (2:1:2) havde den bedste h\u00e6mmende effekt p\u00e5 LPS-induceret inflammation i RAW 264.7-celler, og den ud\u00f8vede antiinflammatoriske virkninger in vitro ved at h\u00e6mme aktiveringen af NF - \u03ba B-inflammationsvejen. Dette eksperiment afsluttede kun screeningen af antiinflammatoriske l\u00e6gemidler p\u00e5 celleniveau og screenede kun kompatibilitetskombinationerne af forskellige farmakologiske dele af Sophora alopecuroides. Der er dog stadig problemer s\u00e5som kompleks kemisk sammens\u00e6tning, uklare farmakologiske komponenter og det materielle grundlag for deres virkninger, som kr\u00e6ver yderligere forskning.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the anti-inflammatory effect and mechanism of Sophora alopecuroides on lipopolysaccharide induced inflammation in RAW264.7 cells Inflammation is a defensive response that occurs when the body is attacked or infected by harmful internal and external stimuli such as pathogens. It triggers an immune response to protect the body. When the immune system is excessively […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the anti-inflammatory effect and mechanism of Sophora alopecuroides on lipopolysaccharide induced inflammation in RAW264.7 cells - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/da\/sophora-alopecuroides\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the anti-inflammatory effect and mechanism of Sophora alopecuroides on lipopolysaccharide induced inflammation in RAW264.7 cells - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the anti-inflammatory effect and mechanism of Sophora alopecuroides on lipopolysaccharide induced inflammation in RAW264.7 cells Inflammation is a defensive response that occurs when the body is attacked or infected by harmful internal and external stimuli such as pathogens. 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