{"id":7433,"date":"2024-08-06T19:50:37","date_gmt":"2024-08-06T19:50:37","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7433"},"modified":"2024-08-06T19:50:37","modified_gmt":"2024-08-06T19:50:37","slug":"x-396","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/da\/x-396\/","title":{"rendered":"Isolering og identifikation af den endofytiske svamp X-396 fra fennikel og analyse af lipidopl\u00f8selige komponenter i dens fermenterede produkt"},"content":{"rendered":"

Isolering og identifikation af den endofytiske svamp X-396 fra fennikel og analyse af lipidopl\u00f8selige komponenter i dens fermenterede produkt
\nPlanteendofytiske svampe refererer til svampe, der ofte er til stede i sundt plantev\u00e6v, men som generelt ikke for\u00e5rsager plantesygdomme. De udvikler sig sammen med v\u00e6rtsplanter for at danne stabile \u00f8kologiske forhold og kan producere bioaktive stoffer, der er de samme eller forskellige fra v\u00e6rtsplantens. Forskning har vist, at n\u00e6sten alle unders\u00f8gte planter indeholder endofytiske svampe, ogs\u00e5 l\u00e6geplanter. Endofytiske svampe i l\u00e6geplanter er en ekstremt rigelig og ny mikrobiel ressource med et stort antal og en bred fordeling. Der er mange typer v\u00e6rtsplanter, og deres forhold til dem er ekstremt komplekst. De kan producere forskellige aktive produkter og har brede udsigter til udvikling og anvendelse. I forbindelse med langsom naturlig regenerering af l\u00e6geplanter, lav udvinding af aktive ingredienser og reduceret biodiversitet for\u00e5rsaget af overdreven menneskelig indgriben er dybdeg\u00e5ende forskning i endofytiske svampe og deres metabolitter i l\u00e6geplanter en af de effektive m\u00e5der at beskytte sj\u00e6ldne l\u00e6geplanter p\u00e5 og opn\u00e5 storstilet produktion af medicinske ingredienser. I de senere \u00e5r har forskere isoleret mange strukturelt nye og meget aktive monomere forbindelser fra endofytiske svampes sekund\u00e6re metabolitter. Naturprodukter fremstillet gennem svampemetabolisme har betydelige fordele i forhold til udbytte, \u00f8konomi og milj\u00f8beskyttelse sammenlignet med l\u00e6geplanter.<\/p>\n

Tibetanske l\u00e6geplanter indeholder p\u00e5 grund af deres v\u00e6kst i h\u00f8jtliggende omr\u00e5der med store temperaturforskelle, lav nedb\u00f8r, lang lyseksponering og st\u00e6rk str\u00e5ling unikke aktive ingredienser og biologiske aktiviteter, hvilket g\u00f8r dem til en vigtig kilde til naturmedicin eller blyforbindelser. Den tibetanske medicin Hypecoum leptocarpum Hook. f. et Thoms. er en t\u00f8rret hel plante af sl\u00e6gten Hypecoum i familien Papaveraceae. Den tibetanske medicin kaldes \"Balbada\", som har en bitter smag og en kold natur. Den har en varmeafledende, afgiftende, antiinflammatorisk og smertestillende virkning. Det bruges hovedsageligt til at behandle influenza, lungebet\u00e6ndelse, hoste, ledsmerter, ondt i halsen, lindre madforgiftning og er velegnet til forskellige infektionssygdomme, der for\u00e5rsager feber. P\u00e5 nuv\u00e6rende tidspunkt fokuserer forskningen i denne l\u00e6geplante hovedsageligt p\u00e5 planteressourcer, kemisk sammens\u00e6tning og biologisk aktivitet, og der er kun lidt opm\u00e6rksomhed p\u00e5 dens endofytiske svampe. Derfor har udvinding af aktive forbindelser fra den stor forskningsv\u00e6rdi og videnskabelig betydning. Denne unders\u00f8gelse isolerede og rensede en endofytisk svamp X-396 fra fennikel og identificerede den. De lipidopl\u00f8selige komponenter i dens fermenteringsprodukt blev analyseret ved hj\u00e6lp af GC-MS-teknologi, og dens antitumoraktivitet blev unders\u00f8gt ved hj\u00e6lp af CCK-8-metoden. Det forventes at give et vist datagrundlag og en referencev\u00e6rdi for effektiv udnyttelse af endofytiske svamperessourcer i fennikel og udvikling af nye antitumoraktive blyforbindelser i fremtiden.<\/p>\n

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\nDenne unders\u00f8gelse analyserede de lipidopl\u00f8selige komponenter i det fermenterede produkt af den endofytiske svamp X-396 i fennikel ved hj\u00e6lp af GC-MS. For at \u00f8ge flygtigheden af dets lipofile komponenter og adskillelsen af lignende komponenter udf\u00f8rte denne unders\u00f8gelse derivatiseringsbehandling f\u00f8r GC-MS-analyse. Resultaterne af GC-MS-analysen viste, at sammenlignet med de 17 lipidopl\u00f8selige komponenter, der blev identificeret i v\u00e6rtsplanten fennikel, var de bioaktive komponenter i de lipidopl\u00f8selige komponenter i det fermenterede produkt af den endofytiske svamp X-396 mere forskelligartede og indeholdt en stor m\u00e6ngde sterolforbindelser. P\u00e5 grund af den lignende struktur af plantesteroler er det vanskeligt at identificere deres typer n\u00f8jagtigt. I denne unders\u00f8gelse blev tre standardpr\u00f8ver af brassinosteroider, stigmasterol og \u03b2-sitosterol udvalgt til derivatisering og GC-MS-detektion, og steroltyperne i de lipidopl\u00f8selige komponenter i X-396-fermenteringsproduktet blev i sidste ende bestemt. Det relative indhold af ergosterol og \u03b2-sitosterol var henholdsvis 31,83% og 12,00%. Ergosterol er en vigtig komponent i svampecellemembraner. P\u00e5 grund af dets stabile struktur og st\u00e6rke specificitet kan dets indhold bruges som en indikator for svampebiomasse og fremme optagelsen af calcium og fosfor i menneskekroppen. Det er en af de gode kilder til eksogent vitamin D2 til menneskekroppen. \u03b2-sitosterol er et sikkert, naturligt, gr\u00f8nt og effektivt kosttilskud til mennesker, der ikke giver genetisk eller cellul\u00e6r toksicitet. Kliniske fors\u00f8g og unders\u00f8gelser af modeldyr har bekr\u00e6ftet, at \u03b2-sitosterol har forskellige biologiske aktiviteter s\u00e5som kolesterols\u00e6nkende, blodsukkers\u00e6nkende, antioxidante, antiinflammatoriske, antibakterielle og kr\u00e6fth\u00e6mmende egenskaber.<\/p>\n

Gennem GC-MS-analyse blev aktive ingredienser som methylhexadecanoat, laktr\u00e6ssyre og squalen identificeret fra X-396-fermenteringsproduktet. Blandt dem er methylhexadecanoat en mide-dr\u00e6bende forbindelse, der kan h\u00e6mme fagocytisk aktivitet og immunrespons. Der er ogs\u00e5 litteraturrapporter om, at det har antiinflammatoriske virkninger. Lacktr\u00e6ssyre har antiinflammatoriske og antiangiogene virkninger. Squalen, som er et forstadie til steroidsyntese, deltager i stofskiftet og spiller en vigtig rolle i syntesevejene for \u03b2-caroten, coenzym Q10, D-vitamin, E-vitamin og K1-vitamin. Derfor kan squalen spille en vigtig rolle i anti-inflammatorisk, antioxidant, hud- og kardiovaskul\u00e6r beskyttelse.<\/p>\n

Med hensyn til in vitro-aktivitetsscreening har de lipidopl\u00f8selige komponenter fra X-396-fermentering bedre antitumoraktivitet mod HepG-celler. Denne unders\u00f8gelse beriger forskningen i sammens\u00e6tningen af endofytiske svampefermenteringsprodukter fra fennikel og giver visse datareferencer og et videnskabeligt grundlag for den efterf\u00f8lgende aktivitetsforskning af endofytiske svampefermenteringsprodukter fra fennikel.<\/p>","protected":false},"excerpt":{"rendered":"

Isolation and identification of endophytic fungus X-396 from fennel and analysis of lipid soluble components in its fermented product Plant endophytic fungi refer to fungi that are commonly present in healthy plant tissues but generally do not cause plant diseases. They co evolve with host plants to form stable ecological relationships and can produce bioactive […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nIsolation and identification of endophytic fungus X-396 from fennel and analysis of lipid soluble components in its fermented product - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/da\/x-396\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Isolation and identification of endophytic fungus X-396 from fennel and analysis of lipid soluble components in its fermented product - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Isolation and identification of endophytic fungus X-396 from fennel and analysis of lipid soluble components in its fermented product Plant endophytic fungi refer to fungi that are commonly present in healthy plant tissues but generally do not cause plant diseases. 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