{"id":7243,"date":"2024-08-06T19:07:24","date_gmt":"2024-08-06T19:07:24","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7243"},"modified":"2024-08-06T19:07:24","modified_gmt":"2024-08-06T19:07:24","slug":"ficus-microcarpa","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/da\/ficus-microcarpa\/","title":{"rendered":"Identifikation af Ficus microcarpa og dens besl\u00e6gtede arter baseret p\u00e5 ITS2-sekvens og HPLC-fingeraftryk"},"content":{"rendered":"

Identifikation af Ficus microcarpa og dens besl\u00e6gtede arter baseret p\u00e5 ITS2-sekvens og HPLC-fingeraftryk
\nFicus pandurata Hance, ogs\u00e5 kendt som m\u00e6lketr\u00e6 eller m\u00e6lkefr\u00f8, er en plante i Ficus-sl\u00e6gten i Moraceae-familien. Den er hovedsageligt udbredt i Guangdong, Guangxi, Jiangxi, Hainan, Fujian, Hunan, Hubei, Jiangxi, Anhui, Zhejiang og andre steder. If\u00f8lge \"Dictionary of Traditional Chinese Medicine\", \"Fujian Medicinal Records\" og \"Jiangxi Folk Herbal Formula Testing\" bruges roden og bladdelene af Ficus microcarpa som medicin. Den har en mild natur, en s\u00f8d og lidt k\u00f8lig smag og er ugiftig. Den fjerner varme, styrker milten, stimulerer appetitten, n\u00e6rer qi og genererer v\u00e6sker samt fjerner fugt og stagnation. Den bruges til at fjerne vind og fugt, afgifte, behandle malaria og fremme amning. For eksempel viser \"Jiangxi Folk Herbal Formula Testing\", at moderate m\u00e6ngder af Ficus microcarpa-r\u00f8dder og Malan kan bruges til at behandle gulsot, n\u00e5r de tages oralt i et afkog med klart vand; En passende m\u00e6ngde banyanrod, Brahma-rod, Stephania tetrandra, Acanthopanax senticosus-rod og malurtstamme kan bruges til at behandle smerter i l\u00e6nden med en blanding af Baijiu og vand af samme volumen. I Guangdong har Ficus microcarpa en lang historie med mad og medicin og en dyb massebase, som er blevet en vigtig del af den lokale karakteristiske Tonic Diet og r\u00e5materialerne til di\u00e6tmedicin. Moderne forskning har vist, at Ficus microcarpa indeholder aktive ingredienser som triterpenoider, flavonoider, coumariner og fenoliske syrer. Blandt dem har bergamotlakton forskellige farmakologiske aktiviteter s\u00e5som antitumor, blodsukkerregulering, forbedring af s\u00f8vnl\u00f8shed, forebyggelse og behandling af osteoporose, anti-aging, antiinflammatoriske og antiallergiske virkninger; Psoralen har farmakologiske virkninger s\u00e5som antiosteoporose, neurobeskyttelse, antitumor, \u00f8strogenlignende, antiinflammatoriske osv. Begge har virkninger mod osteoporose, er rigelige og let tilg\u00e6ngelige og er repr\u00e6sentative aktive ingredienser i Ficus microcarpa.
\nP\u00e5 nuv\u00e6rende tidspunkt findes der tre hovedtyper af blandede produkter af Ficus microcarpa-medicinske urter p\u00e5 markedet: Ficus microcarpa var. holophylla, Ficus microcarpa var. angustifolia og Ficus microcarpa var. hirta. Quanyuan Qinye Ficus og Tiaoye Ficus er varianter af Qinye Ficus med overgangsbladformer mellem de tre; desuden bruger b\u00e5de Ficus microcarpa og Ficus microcarpa deres r\u00f8dder som medicin og har en m\u00e6lkeagtig aroma. De s\u00e6lges ofte p\u00e5 markedet under handelsnavnet Milk Tree Roots, hvilket resulterer i uj\u00e6vn kvalitet af de medicinske materialer. P\u00e5 nuv\u00e6rende tidspunkt fokuserer moderne forskning i Ficus microcarpa hovedsageligt p\u00e5 farmakognosi, kemisk sammens\u00e6tning og farmakologisk aktivitet, og der er ingen rapporter om kvalitetsstandarderne for Ficus microcarpa. DNA-stregkodeidentifikation er en molekyl\u00e6rbiologisk identifikationsmetode, der bruger en standard, relativt kort DNA-sekvens til artsidentifikation. Denne metode er af stor betydning for at fremme udviklingen og udnyttelsen af nye kinesiske medicinressourcer, afhj\u00e6lpe manglen p\u00e5 talenter inden for identifikation af kinesisk medicin, styre cirkulationen af kinesiske medicinmarkeder og l\u00f8se de skjulte fr\u00f8problemer, som morfologi er vanskelig at overvinde. HPLC-fingeraftryk er en p\u00e5lidelig metode til at identificere \u00e6gtheden, evaluere kvalitetskonsistens og stabilitet af kemiske komponenter i traditionel kinesisk medicin opn\u00e5et gennem kromatografiske teknikker baseret p\u00e5 forst\u00e5elsen af den samlede virkning af stofgrupper. I dette eksperiment blev ITS2-sekvenser brugt til molekyl\u00e6r identifikation af Ficus microcarpa og dens besl\u00e6gtede arter. HPLC-fingeraftryksspektre blev etableret for at bestemme forskellene i indholdet af psoralen og bergamotlakton og for at identificere Ficus microcarpa og dens besl\u00e6gtede arter fra forskellige oprindelser. Dette giver et videnskabeligt grundlag for kvalitetskontrol og evaluering af medicinske materialer fra Ficus microcarpa.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"Eksperimentet analyserede ITS2-sekvensen, den genetiske K2P-afstand, NJ-tr\u00e6klyngeanalysen og den sekund\u00e6re struktur af Ficus microcarpa og dens besl\u00e6gtede arter. B\u00e5de NJ-tr\u00e6klyngeanalyse og sekund\u00e6rstruktur kan effektivt skelne mellem Ficus microcarpa og dens besl\u00e6gtede arter. Den genetiske afstand inden for og mellem K2P-arter har dog visse begr\u00e6nsninger i forhold til at identificere Ficus microcarpa og dens besl\u00e6gtede arter og kr\u00e6ver en kombination af NJ-tr\u00e6klusteranalyse og sekund\u00e6r struktur til identifikation.<\/p>\n

Eksperimentet unders\u00f8gte kromatografiske forhold s\u00e5som forskellige ekstraktionsmidler, detektionsb\u00f8lgel\u00e6ngder, s\u00f8jletemperaturer, mobile fasesystemer og kromatografiske s\u00f8jler. Resultaterne viste, at ved brug af 95% ethanolekstraktion, XBridge Peptide BEH C18 (5 \u03bc m, 4,6 mm \u00d7 250 mm) kromatografisk s\u00f8jle, detektionsb\u00f8lgel\u00e6ngde 330 nm, s\u00f8jletemperatur 30 \u00b0C, str\u00f8mningshastighed 1,0 ml\/min og mobilfase methanol-pH8 fosfatbufferopl\u00f8sning var topformerne og separationsgraden for hvert farvespektrum gode. Vi har etableret HPLC-fingeraftryksspektre af Ficus microcarpa og dens besl\u00e6gtede arter, hvilket udfylder hullet i unders\u00f8gelsen af HPLC-karakteristiske spektre af Ficus microcarpa. Fingeraftryksspektrene for Ficus microcarpa og dens besl\u00e6gtede arter viser betydelige forskelle i antallet af karakteristiske toppe og relativ retentionstid, som intuitivt kan skelne Ficus microcarpa fra dens besl\u00e6gtede arter og anvendes til kvalitetsevaluering af Ficus microcarpa. Der er i alt 10 kromatografiske toppe i Ficus microcarpa fra forskellige oprindelser, hvoraf de fleste ikke blev p\u00e5vist i Ficus microcarpa og Ficus microcarpa. Ligheden mellem n\u00e6rt besl\u00e6gtede arter og Ficus microcarpa er mindre end 0,807; der er en betydelig forskel i indholdet af psoralen og bergamotlakton mellem Ficus microcarpa og dens besl\u00e6gtede arter. Indholdet af Ficus microcarpa og Ficus microcarpa er betydeligt lavere end indholdet af Ficus microcarpa, hvilket indikerer en forskel i kvalitet mellem Ficus microcarpa og dens besl\u00e6gtede arter. Det har en vigtig praktisk vejledningsv\u00e6rdi for brugen af indholdet af psoralen og bergamotlakton som kvalitetskontrolindikatorer for Ficus microcarpa. Derfor kan fingeraftryksspektre, lighed og indholdsbestemmelse objektivt og pr\u00e6cist skelne mellem \u00e6gtheden af Ficus microcarpa-l\u00e6gemidler, som effektivt kan bruges til kvalitetskontrol og \u00e6gthedsidentifikation af Ficus microcarpa-l\u00e6gemidler.<\/p>\n

Sammenfattende er DNA-stregkoden og HPLC-fingeraftrykket for Ficus microcarpa og dens besl\u00e6gtede arter blevet etableret. Metoden er enkel, p\u00e5lidelig, stabil og har god repeterbarhed, hvilket kan bruges til fingeraftryksforskning og autenticitetsidentifikation af Ficus microcarpa og dens besl\u00e6gtede arter. Samtidig giver den et videnskabeligt grundlag for yderligere at forbedre kvaliteten af Ficus microcarpa.
\nI PPI-netv\u00e6rket blev der udvalgt 42 vigtige m\u00e5l baseret p\u00e5 gradrangering, som hovedsageligt blev opdelt i 5 kategorier: proteintyrosinfosfataser (PTPN1, PTPN2, PTPN6, PTPN11 osv.), phosphatidylinositol 3-kinaser (PIK3R1, PIK3CB, PIK3CA osv.), mitogenaktiverede proteinkinaser (MAPK1, MAPK3, MAPK8, MAPK14 osv.), cyclinafh\u00e6ngige kinaser (CDK1, CDK2, CDK4 osv.) og andre vigtige m\u00e5l (VEGFA, HSP90AA1, LCK osv.). Disse vigtige m\u00e5l kan fungere som reference for m\u00e5lrettet behandling af UC.<\/p>","protected":false},"excerpt":{"rendered":"

Identification of Ficus microcarpa and its related species based on ITS2 sequence and HPLC fingerprint The Ficus pandurata Hance, also known as the milk tree or milk seed, is a plant in the Ficus genus of the Moraceae family. It is mainly distributed in Guangdong, Guangxi, Jiangxi, Hainan, Fujian, Hunan, Hubei, Jiangxi, Anhui, Zhejiang and […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nIdentification of Ficus microcarpa and its related species based on ITS2 sequence and HPLC fingerprint - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/da\/ficus-microcarpa\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Identification of Ficus microcarpa and its related species based on ITS2 sequence and HPLC fingerprint - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Identification of Ficus microcarpa and its related species based on ITS2 sequence and HPLC fingerprint The Ficus pandurata Hance, also known as the milk tree or milk seed, is a plant in the Ficus genus of the Moraceae family. 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