{"id":4983,"date":"2024-08-04T16:44:55","date_gmt":"2024-08-04T16:44:55","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4983"},"modified":"2024-08-04T16:44:55","modified_gmt":"2024-08-04T16:44:55","slug":"turtle-shell-glue","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/da\/lim-til-skildpaddeskjold\/","title":{"rendered":"Unders\u00f8gelse af forskellene i aminosyreindhold og potentielle mekanismer mod osteoporose i skildpaddekl\u00e6bemiddel fra forskellige producenter baseret p\u00e5 UHPLC-QTRAP-MS\/MS kombineret med netv\u00e6rksfarmakologi"},"content":{"rendered":"<p>Unders\u00f8gelse af forskellene i aminosyreindhold og potentielle mekanismer mod osteoporose i skildpaddekl\u00e6bemiddel fra forskellige producenter baseret p\u00e5 UHPLC-QTRAP-MS\/MS kombineret med netv\u00e6rksfarmakologi<br \/>\nTestudinis Carapacis et Plasti Colla (TCPC) er en traditionel kinesisk n\u00e6rende medicin, som er en fast gel fremstillet ved at koge og koncentrere skildpaddeskjoldet fra skildpaddefamilien Chinemys reevesii (Gray) i vand. Det blev f\u00f8rste gang registreret i \"Compendium of Materia Medica\". Den har en k\u00f8lig natur, en salt og s\u00f8d smag og er velegnet til lever-, nyre- og hjertemeridianerne. Den bruges ofte til behandling af osteoporose i klinisk praksis. Klassiske formler, der hovedsageligt best\u00e5r af lim fra skildpaddeskjold, s\u00e5som formlen i Guilu Erxian-serien, Zuogui Wan, Huqian Wan osv., har betydelige virkninger, n\u00e5r det g\u00e6lder om at give n\u00e6ring til essensen, fylde marven og styrke sener og knogler. Lim fra skildpaddeskjold er rig p\u00e5 kollagen, keratin og frie aminosyrer. Aminosyrerne fra proteinhydrolyse er de vigtigste aktive ingredienser i lim fra skildpaddeskjold, som i sidste ende absorberes og udnyttes af menneskekroppen. I den traditionelle kinesiske medicins diagnose og behandling klassificeres osteoporose for det meste som \"knogleobstruktion\", \"knoglenekrose\" og \"knoglefistel\". I \"Essential Prescriptions for Urgent Care\" st\u00e5r der: \"For dem med ekstreme knogletilstande ... kan de opleve smerter i h\u00e6nder og f\u00f8dder, manglende evne til at st\u00e5 i lang tid og vanskeligheder med at b\u00f8je og str\u00e6kke sig, hvilket kan f\u00f8re til kropsobstruktion, hjerne- og rygmarvsdysfunktion. Nyrerne opbevarer essensen og kontrollerer knoglemarvsproduktionen. Hvis den er utilstr\u00e6kkelig, vil knoglemarven falde, og knoglen vil atrofiere. Tabet af knoglemarv vil f\u00f8re til \u00f8get knoglesk\u00f8rhed og osteoporose. Moderne forskning har vist, at skildpaddeskalslim og dets klassiske formler har gode aktiviteter inden for knoglesundhed, immunforbedring, antitumor og andre aspekter. Glycin og prolin i skildpaddeskalslim kan fungere som potentielle biomark\u00f8rer til diagnosticering af postmenopausal osteoporose. Aminosyrer er i sig selv signalmolekyler og kan ogs\u00e5 synergistisk aktivere calciumf\u00f8lsomme receptorer med calciumioner, \u00f8ge knoglet\u00e6theden, fremme osteoblastdifferentiering og forbedre knogleforkalkningen. For at unders\u00f8ge forskellene i aminosyreindholdet i lim fra skildpaddeskjold fra forskellige producenter blev der i denne unders\u00f8gelse udvalgt 15 partier lim fra skildpaddeskjold fra 4 forskellige producenter. V\u00e6skekromatografi-massespektrometri blev brugt til at bestemme 17 aminosyrer efter syrehydrolyse af lim fra skildpaddeskjold. Multivariat statistik blev brugt til at sammenligne forskelle i sammens\u00e6tningen af hydrolyserede aminosyrekomponenter i lim fra skildpaddeskjold fra forskellige producenter, hvilket giver reference til kvalitetskontrol af lim fra skildpaddeskjold. Og kombineret med netv\u00e6rksfarmakologi til at analysere aminosyrekomponenterne i skildpaddeskalslim, hvilket giver et grundlag for yderligere at forudsige de vigtigste aktive ingredienser og m\u00e5l for skildpaddeskalslim i antiosteoporose.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4984\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-108.png\" alt=\"\" width=\"782\" height=\"418\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-108.png 782w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-108-768x411.png 768w\" sizes=\"(max-width: 782px) 100vw, 782px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4985\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-94.png\" alt=\"\" width=\"778\" height=\"320\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-94.png 778w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-94-768x316.png 768w\" sizes=\"(max-width: 778px) 100vw, 778px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4986\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-90.png\" alt=\"\" width=\"781\" height=\"482\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-90.png 781w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-90-768x474.png 768w\" sizes=\"(max-width: 781px) 100vw, 781px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4987\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-79.png\" alt=\"\" width=\"776\" height=\"249\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-79.png 776w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-79-768x246.png 768w\" sizes=\"(max-width: 776px) 100vw, 776px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4988\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-73.png\" alt=\"\" width=\"778\" height=\"498\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-73.png 778w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-73-768x492.png 768w\" sizes=\"(max-width: 778px) 100vw, 778px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4989\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-65.png\" alt=\"\" width=\"776\" height=\"275\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-65.png 776w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-65-768x272.png 768w\" sizes=\"(max-width: 776px) 100vw, 776px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4990\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-61.png\" alt=\"\" width=\"779\" height=\"285\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-61.png 779w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-61-768x281.png 768w\" sizes=\"(max-width: 779px) 100vw, 779px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4991\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-55.png\" alt=\"\" width=\"780\" height=\"425\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-55.png 780w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-55-768x418.png 768w\" sizes=\"(max-width: 780px) 100vw, 780px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4992\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-49.png\" alt=\"\" width=\"766\" height=\"241\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4993\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-42.png\" alt=\"\" width=\"779\" height=\"453\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-42.png 779w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-42-768x447.png 768w\" sizes=\"(max-width: 779px) 100vw, 779px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4994\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-36.png\" alt=\"\" width=\"776\" height=\"748\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-36.png 776w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-36-768x740.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-36-747x720.png 747w\" sizes=\"(max-width: 776px) 100vw, 776px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4995\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-32.png\" alt=\"\" width=\"779\" height=\"838\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-32.png 779w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-32-768x826.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-32-669x720.png 669w\" sizes=\"(max-width: 779px) 100vw, 779px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4996\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-27.png\" alt=\"\" width=\"780\" height=\"311\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-27.png 780w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-27-768x306.png 768w\" sizes=\"(max-width: 780px) 100vw, 780px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4997\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-23.png\" alt=\"\" width=\"780\" height=\"146\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-23.png 780w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-23-768x144.png 768w\" sizes=\"(max-width: 780px) 100vw, 780px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4998\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-17.png\" alt=\"\" width=\"777\" height=\"330\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-17.png 777w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-17-768x326.png 768w\" sizes=\"(max-width: 777px) 100vw, 777px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4999\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-14.png\" alt=\"\" width=\"779\" height=\"308\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-14.png 779w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-14-768x304.png 768w\" sizes=\"(max-width: 779px) 100vw, 779px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5000\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-12.png\" alt=\"\" width=\"778\" height=\"300\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-12.png 778w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-12-768x296.png 768w\" sizes=\"(max-width: 778px) 100vw, 778px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5001\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/18-10.png\" alt=\"\" width=\"782\" height=\"303\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/18-10.png 782w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/18-10-768x298.png 768w\" sizes=\"(max-width: 782px) 100vw, 782px\" \/>Det samlede aminosyreindhold i hydrolyseret skildpaddeskalslim fra forskellige producenter og partier i denne artikel er lavere end farmakop\u00e9niveauet. \u00c5rsagen til dette kan ikke kun v\u00e6re relateret til kvaliteten af selve pr\u00f8ven, men ogs\u00e5 til testmetoden. Vi bruger v\u00e6skekromatografi-massespektrometri til at detektere de karakteristiske ionpar af hydrolyserede aminosyrer, som har h\u00f8j f\u00f8lsomhed og specificitet; HPLC-metoden, der anvendes i farmakop\u00e9en, detekterer absorbanssignalerne fra karakteristiske funktionelle grupper af forbindelser, hvilket g\u00f8r det vanskeligt at skelne forbindelser med lignende strukturer. V\u00e6skekromatografi-massespektrometri (LC-MS)-metoden er mere pr\u00e6cis og kan eliminere interferens fra ikke-specifikke komponenter, s\u00e5 indholdet af hydrolyserede aminosyrer kan v\u00e6re lavere end i farmakop\u00e9en. Resultaterne af netv\u00e6rksfarmakologi viser, at n\u00f8glekomponenterne i skildpaddeskalslim til behandling af osteoporose er Ala og Gly. Ala kan hj\u00e6lpe med metabolismen af sukker og organiske syrer og forbedre postmenopausal osteoporose. Gly er direkte relateret til risikoen for knoglebrud hos m\u00e6nd, og mutationer i kollagen kan for\u00e5rsage osteogenesis imperfecta. De vigtigste m\u00e5l for behandling af osteoporose med skildpaddeskalslim omfatter CAT, NOS1, NOS3, F2 osv. Catalase (CAT) kan \u00f8ge knoglet\u00e6theden og knoglemineralindholdet i mus ved at h\u00e6mme oxidativ stress; Neuronal nitrogenoxidsyntase (NOS1) er forbundet med knogleheling og udtrykkes hovedsageligt i fibrocartilage-regionen mellem fibr\u00f8st v\u00e6v og callus; Endotelial nitrogenoxidsyntase (NOS3) er ogs\u00e5 forbundet med knogleheling og udtrykkes hovedsageligt i kondrocytter og perivaskul\u00e6re celler; Prothrombin er en specifik agonist i osteoblaster, der kan stimulere knogleresorption i organkultur. Resultaterne af KEGG- og GO-berigelsesanalysen antyder, at skildpaddegummi kan v\u00e6re involveret i en r\u00e6kke biologiske funktioner s\u00e5som vitaminbinding, pyridoxalfosfatbinding, organisk syrebinding og aminosyresyntesemetabolisme i knogleceller; Det fungerer ved at p\u00e5virke signalveje s\u00e5som insulinsignalering, calciumsignalering, cyklisk guanosinmonofosfatproteinkinase G (cGMP PKG) -signalering, kulstofmetabolisme og aminosyresyntese. Insulinsignalvejen kan stimulere skeletmuskler til at optage 80% af kroppens glukose; cGMP PKG-signalvejen kan regulere knogledannelse og fremme osteoblastmineralisering og -modning, hvilket udviser en tovejs og kompleks regulering af osteogenese.<br \/>\nSammenfattende bruger denne artikel UHPLC-QTRAP-MS \/ MS kombineret med multivariat statistisk analyse for at finde ud af, at blandt de fire producenter har en producent stabil kvalitet af skildpaddeskallim og h\u00f8jt indhold af hydrolyserede aminosyrer; Skildpaddeskallim kan regulere 68 kernem\u00e5l for osteoporose, herunder CAT, NOS1, NOS3 osv, gennem komponenter som alanin og glycin og derved p\u00e5virke 212 signalveje s\u00e5som aminosyresyntese, arginin- og prolinmetabolisme og kulstofmetabolisme for at ud\u00f8ve terapeutiske virkninger p\u00e5 osteoporose.<\/p>","protected":false},"excerpt":{"rendered":"<p>Exploring the Differences in Amino Acid Content and Potential Mechanisms of Anti Osteoporosis in Turtle Shell Adhesive from Different Manufacturers Based on UHPLC-QTRAP-MS\/MS Combined with Network Pharmacology Testudinis Carapacis et Plasti Colla (TCPC) is a traditional Chinese nourishing medicine, which is a solid gel made by boiling and concentrating the turtle shell of the turtle [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - 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