{"id":3578,"date":"2024-08-02T15:11:15","date_gmt":"2024-08-02T15:11:15","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=3578"},"modified":"2024-08-02T15:16:02","modified_gmt":"2024-08-02T15:16:02","slug":"the-sugar-components-of-fern-based-on-pmp-pre-column-derivatization-hplc-method","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/da\/the-sugar-components-of-fern-based-on-pmp-pre-column-derivatization-hplc-method\/","title":{"rendered":"Unders\u00f8gelse af effekten af dampeksplosionsbehandling p\u00e5 sukkerkomponenterne i bregner baseret p\u00e5 PMP pre column derivatization HPLC-metode"},"content":{"rendered":"

Unders\u00f8gelse af effekten af dampeksplosionsbehandling p\u00e5 sukkerkomponenterne i bregner baseret p\u00e5 PMP pre column derivatization HPLC-metode
\nPteridium aquilinum, en plante i Pteridium-sl\u00e6gten i Pteridiaceae-familien, er vidt udbredt og har st\u00e6rke s\u00e6sonvariationer. Den h\u00f8stes fra marts til juni hvert \u00e5r og er tilb\u00f8jelig til at \u00e6ldes og forringes efter h\u00f8st. Traditionelle forarbejdningsmetoder bruger ofte bejdsning og t\u00f8rring, hvilket f\u00f8rer til et enkelt udvalg af bregneprodukter p\u00e5 markedet, som kr\u00e6ver tilberedning f\u00f8r indtagelse, hvilket begr\u00e6nser markedsf\u00f8ringen af bregneprodukter. For at fremme udviklingen og udnyttelsen af bregneressourcer og samtidig sikre n\u00e6ringsv\u00e6rdien og sammens\u00e6tningen af selve bregnen s\u00e5 meget som muligt er der i de senere \u00e5r blevet udviklet en r\u00e6kke bregneprodukter med h\u00f8j v\u00e6rditilv\u00e6kst ved at kombinere avancerede forarbejdnings- og opbevaringsteknologier i ind- og udland, herunder papirbregne, blancheret bregne og dampet bregne-te. Blandt dem har dampet bregne-te en enkel forarbejdningsteknologi, praktisk og hurtigt forbrug og er mere befordrende for industrialisering og produktfremme. Dampeksplosionsteknologi (kaldet \"dampeksplosion\") henviser til en termisk mekanisk behandlingsmetode, der placerer materialer i et milj\u00f8 med h\u00f8j temperatur og h\u00f8jt tryk, bruger den genererede vanddamp til at tr\u00e6nge ind i det indre af materialet, og efter at det indre og ydre tryk er afbalanceret, placerer det \u00f8jeblikkeligt alle materialer direkte under atmosf\u00e6risk tryk for at frembringe et \"eksplosions\"-f\u00e6nomen. Denne teknologi kan effektivt nedbryde bregnernes cellev\u00e6gsbarriere, s\u00e5 de aktive ingredienser kan opl\u00f8ses mere effektivt og forkorte bryggetiden betydeligt. Nogle af aktiviteterne i plantematerialer, der er behandlet med denne teknologi, s\u00e5som antioxidant, antibakteriel og h\u00e6mning af alfa-glucosidase, er v\u00e6sentligt forbedret.<\/p>\n

Hele bregneplanten kan bruges som medicin og er rig p\u00e5 forskellige aktive ingredienser som polysaccharider, flavonoider, terpener osv. Blandt dem udg\u00f8r polysaccharider ca. 8% af bregnernes t\u00f8rv\u00e6gt og er en af de vigtigste aktive ingredienser. Pteridium aquilinum-polysaccharid er et heteropolysaccharid med en kompleks k\u00e6destruktur, flere grene og ingen triple helix-struktur. Det har biologiske aktiviteter som immunregulering, antibakteriel aktivitet og regulering af tarmens mikrobiota. Unders\u00f8gelser har vist, at strukturen af polysaccharider har en betydelig indvirkning p\u00e5 deres biologiske aktivitet. Under gaseksplosionsprocessen gennemg\u00e5r komponenter som cellulose og hemicellulose i planter\u00e5varer hydrolyse for at producere opl\u00f8selige sukkerarter, mens reducerende sukkerarter og aminosyrer gennemg\u00e5r Maillard-reaktion, hvilket for\u00e5rsager \u00e6ndringer i sukkerkomponenternes struktur. P\u00e5 nuv\u00e6rende tidspunkt omfatter de almindeligt anvendte analysemetoder for kulhydratkomponenter tyndlagskromatografi, kapillarelektroforese og h\u00f8jtydende v\u00e6skekromatografi. Blandt dem har High Performance Liquid Chromatography (HPLC) god stabilitet, moden teknologi og en bred vifte af kromatografiske s\u00f8jler at v\u00e6lge imellem. P\u00e5 grund af den svage UV-absorption af kulhydratkomponenter kr\u00e6ves der dog normalt derivatiseringsreagenser for at danne derivater med st\u00e6rk UV-absorption. For eksempel kan derivatiseringsreagenset 1-phenyl-3-methyl-5-pyrazolon (PMP) reagere med reducerende sukkerarter for at producere st\u00e6rk UV-absorption. Denne metode er enkel at betjene, meget f\u00f8lsom og n\u00f8jagtig og bruges i vid udstr\u00e6kning til analyse af kulhydratkomponenter.
\nI de senere \u00e5r har indenlandsk og udenlandsk forskning kun rapporteret om \u00e6ndringer i ekstraktionshastighederne for aktive ingredienser som polysaccharider, flavonoider, alkaloider osv. i planter\u00e5varer f\u00f8r og efter dampeksplosion, men der er ikke foretaget nogen unders\u00f8gelse af virkningerne af sukkerkomponenter i bregner f\u00f8r og efter dampeksplosion. I lyset af dette indsamlede denne unders\u00f8gelse t\u00f8rrede pr\u00f8ver af bregner fra fire forskellige produktionsomr\u00e5der og sammenlignede det samlede polysaccharidudbytte efter behandling med den samme dampeksplosionsproces. PMP pre column derivatization HPLC-metoden blev brugt til at etablere et fingeraftryksspektrum, og den hierarkiske klyngeanalyse (HCA) og principal component analysis (PCA) metoder blev brugt i kombination med lighedsevaluering til at identificere de eksperimentelle data og evaluere effekten af dampeksplosionsprocessen p\u00e5 bregnekomponenter. Fem monosaccharidkomponenter blev identificeret, og det frie monosaccharidindhold og forskelle i polysaccharidmonosaccharidsammens\u00e6tningen i hver pr\u00f8ve blev bestemt for at unders\u00f8ge effekten af dampeksplosionsprocessen p\u00e5 sukkerkomponenterne i bregner, hvilket giver et grundlag for efterf\u00f8lgende aktivitetsforskning og kvalitetskontrol af dampeksplosionsbehandling af bregner. Reference.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"I denne unders\u00f8gelse blev der brugt t\u00f8rrede bregnepr\u00f8ver fra fire forskellige produktionsomr\u00e5der som r\u00e5materiale. Efter den samme dampeksplosionsproces blev det samlede polysaccharidudbytte af pr\u00f8verne testet. Det viste sig, at det samlede polysaccharidudbytte af dampeksplosionspr\u00f8ver af bregner steg markant (P<0,05) med 55% sammenlignet med t\u00f8rrede bregner, hvilket bekr\u00e6fter, at dampeksplosionsbehandling kan forbedre polysaccharidudbyttet og opn\u00e5 bregnepolysaccharider mere effektivt. HPLC-teknologi kombineret med PMP-pr\u00e6kolonnederivatisering blev brugt til at etablere et fingeraftryksspektrum, og HCA- og PCA-metoder blev brugt til at analysere r\u00e5dataene. Det viste sig, at der ikke var nogen signifikant \u00e6ndring i antallet af kromatografiske toppe for begge, og der blev identificeret 12 f\u00e6lles toppe. HCA- og PCA-metoder kan effektivt skelne mellem t\u00f8r forarbejdning og dampeksplosionsbehandling af bregner; Samtidig identificering af 5 typer sukkerkomponenter til bestemmelse af fri monosaccharid og monosaccharidsammens\u00e6tning viste resultaterne, at sammenlignet med t\u00f8rret bregne faldt indholdet af frie monosaccharider i dampet bregne, herunder galactose, mannose og xylose, mens indholdet af glukose og arabinose steg; Den gennemsnitlige andel af glukose og xylose i monosaccharidsammens\u00e6tningen af polysaccharider steg markant (P<0.01) med stigninger p\u00e5 henholdsvis 1,64% og 0,94%. Gennem denne unders\u00f8gelse blev det konstateret, at dampeksplosionsbehandling har en vis indvirkning p\u00e5 indholdet og strukturen af sukkerkomponenter i bregner eller kan for\u00e5rsage \u00e6ndringer i deres aktivitet. Dette kan give reference til efterf\u00f8lgende aktivitetsforskning og kvalitetskontrol af dampeksplosionsbehandling af bregner.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the effect of steam explosion processing on the sugar components of fern based on PMP pre column derivatization HPLC method Pteridium aquilinum, a plant in the Pteridium genus of the Pteridiaceae family, is widely distributed and has strong seasonality. It is harvested from March to June each year and is prone to aging […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the effect of steam explosion processing on the sugar components of fern based on PMP pre column derivatization HPLC method - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/da\/the-sugar-components-of-fern-based-on-pmp-pre-column-derivatization-hplc-method\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the effect of steam explosion processing on the sugar components of fern based on PMP pre column derivatization HPLC method - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the effect of steam explosion processing on the sugar components of fern based on PMP pre column derivatization HPLC method Pteridium aquilinum, a plant in the Pteridium genus of the Pteridiaceae family, is widely distributed and has strong seasonality. 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