{"id":10658,"date":"2026-03-10T12:24:09","date_gmt":"2026-03-10T12:24:09","guid":{"rendered":"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/"},"modified":"2026-03-10T12:24:09","modified_gmt":"2026-03-10T12:24:09","slug":"brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/da\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/","title":{"rendered":"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor"},"content":{"rendered":"

Brefeldin A (BFA)<\/strong> is a powerful fungal metabolite that has become an indispensable tool in modern cell biology and cancer research. With the CAS registry number 20350-15-6<\/strong>, this macrocyclic lactone is produced by the fungus Penicillium brefeldianum<\/em> and has captured the attention of researchers worldwide for its unique ability to interfere with fundamental cellular processes.<\/p>

Originally discovered in the 1950s, Brefeldin A was initially investigated as a potential antibiotic. However, researchers soon realized that its true value lay not in clinical applications, but as a powerful research tool<\/strong> for understanding cellular protein transport mechanisms. Today, it is widely used in laboratories around the world to study intracellular trafficking, apoptosis, and various disease mechanisms.<\/p>

What is Brefeldin A?<\/h2>

Brefeldin A is a macrocyclic lactone with the molecular formula C\u2081\u2086H\u2082\u2084O\u2084 and a molecular weight of 280.36 g\/mol. This white to off-white crystalline powder is produced by the fungus Penicillium brefeldianum<\/em> and is known by various alternative names including BFA, Ascotoxin, Cyanein, Decumbin, and Nectrolide.<\/p>

With a purity typically \u226598%, Brefeldin A is soluble in ethanol, DMSO, and methanol, making it suitable for various laboratory applications in cell biology research.<\/p>

Mechanism of Action: How Brefeldin A Works<\/h2>

The primary mechanism of Brefeldin A centers on its ability to inhibit protein transport<\/strong> from the endoplasmic reticulum (ER) to the Golgi apparatus. This action occurs through several key pathways:<\/p>

1. Inhibition of ADP-Ribosylation Factor (ARF)<\/h3>

Brefeldin A works by inhibiting ADP-ribosylation factor (ARF) GTPases<\/strong>, which are essential for recruiting coat proteins to cellular membranes. These coat proteins (specifically COPI) are necessary for forming transport vesicles that carry proteins from the ER to the Golgi.<\/p>

2. Disruption of Golgi Structure<\/h3>

When cells are treated with Brefeldin A, the Golgi apparatus rapidly disassembles and merges with the endoplasmic reticulum. This dramatic structural change provides researchers with a visual indicator of the compound’s activity and allows for detailed studies of cellular organization.<\/p>

3. Induction of Endoplasmic Reticulum Stress<\/h3>

By preventing protein transport, Brefeldin A causes proteins to accumulate in the ER, leading to ER stress<\/strong>. This stress triggers the unfolded protein response (UPR), which can ultimately lead to apoptosis<\/strong> (programmed cell death).<\/p>

Key Applications and Uses<\/h2>

1. Cell Biology Research<\/h3>

Brefeldin A is primarily used as a research tool<\/strong> in cell biology:<\/p>

  • Protein Transport Studies:<\/strong> Researchers use BFA to understand how proteins move within cells<\/li>
  • Golgi Function Analysis:<\/strong> The compound helps scientists study Golgi apparatus structure and function<\/li>
  • Vesicle Trafficking Research:<\/strong> BFA provides insights into how vesicles form and transport cellular cargo<\/li><\/ul>

    2. Cancer Research and Therapy<\/h3>

    One of the most promising applications of Brefeldin A is in cancer research<\/strong>:<\/p>

    • Apoptosis Induction:<\/strong> BFA induces apoptosis (cell death) in various cancer cell lines<\/li>
    • p53-Independent Action:<\/strong> Unlike many chemotherapeutic agents, BFA can induce apoptosis independently of p53 status, making it potentially useful for treating p53-mutant tumors<\/li>
    • Synergistic Effects:<\/strong> Studies show BFA can enhance the effects of other chemotherapy agents<\/li><\/ul>

      3. Antiviral Research<\/h3>

      Research has demonstrated that Brefeldin A exhibits antiviral properties<\/strong>:<\/p>

      • The compound can inhibit replication of various viruses<\/li>
      • It interferes with viral protein processing and transport<\/li>
      • BFA is used in antiviral drug development research<\/li><\/ul>

        4. Immunology Studies<\/h3>

        In immunological research, Brefeldin A is used to:<\/p>

        • Study cytokine secretion pathways<\/li>
        • Analyze immune cell function<\/li>
        • Investigate antigen presentation mechanisms<\/li><\/ul>

          5. CRISPR\/Cas9 Activation<\/h3>

          Recent research has revealed that Brefeldin A can serve as a CRISPR\/Cas9 activator<\/strong>, enhancing the efficiency of gene editing experiments.<\/p>

          Research Findings and Clinical Potential<\/h2>

          Anticancer Activity<\/h3>

          Multiple studies have demonstrated Brefeldin A’s potential anticancer properties:<\/p>

          • Prostate Cancer:<\/strong> BFA inhibits cell growth by targeting the cell cycle in prostate cancer cells<\/li>
          • Neuroendocrine Tumors:<\/strong> Research shows BFA induces apoptosis through caspase-3 activation<\/li>
          • Various Cancer Lines:<\/strong> Studies demonstrate cytotoxicity against multiple cancer cell lines<\/li><\/ul>

            ER Stress and Apoptosis<\/h3>

            The relationship between BFA-induced ER stress and apoptosis has been extensively studied:<\/p>

            • Protein accumulation in the ER triggers the unfolded protein response<\/li>
            • Prolonged ER stress leads to apoptotic cell death<\/li>
            • Mitochondrial pathways are involved in BFA-induced apoptosis<\/li><\/ul>

              Sikkerhed og h\u00e5ndtering<\/h2>

              Important Note:<\/strong> Brefeldin A is sold exclusively for research and laboratory purposes<\/strong>. It is not intended for diagnostic, therapeutic, or clinical applications in humans or animals.<\/p>

              Sikkerhedsforanstaltninger<\/h3>

              When handling Brefeldin A in a research setting:<\/p>

              • Use appropriate personal protective equipment (PPE)<\/li>
              • Work in a fume hood or biosafety cabinet<\/li>
              • Follow institutional safety guidelines<\/li>
              • Store according to manufacturer recommendations<\/li><\/ul>

                Opbevaring<\/h3>
                • Store at -20\u00b0C or below<\/li>
                • Protect from light<\/li>
                • Keep container tightly sealed<\/li><\/ul>

                  Chemical Specifications<\/h2>
                  CAS-nummer<\/strong><\/td>20350-15-6<\/td><\/tr>
                  Molekyl\u00e6r formel<\/strong><\/td>C\u2081\u2086H\u2082\u2084O\u2084<\/td><\/tr>
                  Molekylv\u00e6gt<\/strong><\/td>280.36 g\/mol<\/td><\/tr>
                  Udseende<\/strong><\/td>Hvidt krystallinsk pulver<\/td><\/tr>
                  Renhed<\/strong><\/td>\u226598%<\/td><\/tr>
                  Opl\u00f8selighed<\/strong><\/td>Ethanol, DMSO, Methanol<\/td><\/tr>
                  Alternative Names<\/strong><\/td>BFA, Ascotoxin, Cyanein, Decumbin, Nectrolide<\/td><\/tr><\/tbody><\/table><\/figure>

                  Why Choose Our Brefeldin A?<\/h2>
                  • High Purity:<\/strong> Our Brefeldin A meets or exceeds research grade specifications (\u226598%)<\/li>
                  • Quality Tested:<\/strong> Each batch is quality tested for purity and biological activity<\/li>
                  • Reliable Supply:<\/strong> Consistent availability for ongoing research needs<\/li>
                  • Technical Support:<\/strong> Our team of scientists is available to answer your questions<\/li>
                  • Competitive Pricing:<\/strong> Factory-direct pricing for research institutions<\/li><\/ul>

                    We serve pharmaceutical companies, contract manufacturers, and research institutions worldwide with premium chemical products and dedicated customer support.<\/p>

                    Frequently Asked Questions<\/h2>

                    What is the primary use of Brefeldin A?<\/h3>

                    The primary use of Brefeldin A is as a research tool<\/strong> in cell biology to study protein transport mechanisms. It is used extensively in laboratories to understand how proteins move within cells and to study cellular processes like apoptosis.<\/p>

                    How does Brefeldin A induce apoptosis?<\/h3>

                    Brefeldin A induces apoptosis by causing endoplasmic reticulum stress<\/strong> through the inhibition of protein transport. This leads to the accumulation of misfolded proteins, triggering the unfolded protein response and ultimately cell death.<\/p>

                    Is Brefeldin A safe for human use?<\/h3>

                    No. Brefeldin A is research-grade only<\/strong> and not approved for human or animal therapeutic use. It is a potent compound that can cause significant cellular effects and should only be used by trained researchers in appropriate laboratory settings.<\/p>

                    What concentration of Brefeldin A is typically used?<\/h3>

                    In cell culture experiments, typical working concentrations range from 0.1 to 10 \u03bcg\/mL<\/strong>, depending on the specific application and cell type. Researchers should optimize conditions for their specific experiments.<\/p>

                    Does Brefeldin A work on all cell types?<\/h3>

                    The effects of Brefeldin A can vary between different cell types. Some cells are more sensitive to BFA-induced apoptosis than others, and the response can depend on factors like p53 status and cellular stress response pathways.<\/p>Disclaimer:<\/strong> This article is for informational purposes only and is intended for research and educational use. Brefeldin A is not for human or animal diagnostic or therapeutic use.<\/em>","protected":false},"excerpt":{"rendered":"

                    Brefeldin A (BFA) is a powerful fungal metabolite that has become an indispensable tool in modern cell biology and cancer research. With the CAS registry number 20350-15-6, this macrocyclic lactone is produced by the fungus Penicillium brefeldianum and has captured the attention of researchers worldwide for its unique ability to interfere with fundamental cellular processes. […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[15],"tags":[],"yoast_head":"\nBrefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/da\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/\" \/>\n<meta property=\"og:locale\" content=\"da_DK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Brefeldin A (BFA) is a powerful fungal metabolite that has become an indispensable tool in modern cell biology and cancer research. With the CAS registry number 20350-15-6, this macrocyclic lactone is produced by the fungus Penicillium brefeldianum and has captured the attention of researchers worldwide for its unique ability to interfere with fundamental cellular processes. […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangextracts.com\/da\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/\" \/>\n<meta property=\"og:site_name\" content=\"China Chemical Manufacturer\" \/>\n<meta property=\"article:published_time\" content=\"2026-03-10T12:24:09+00:00\" \/>\n<meta name=\"author\" content=\"longcha9\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Skrevet af\" \/>\n\t<meta name=\"twitter:data1\" content=\"longcha9\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimeret l\u00e6setid\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutter\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/\",\"url\":\"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/\",\"name\":\"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor - China Chemical Manufacturer\",\"isPartOf\":{\"@id\":\"https:\/\/longchangextracts.com\/#website\"},\"datePublished\":\"2026-03-10T12:24:09+00:00\",\"dateModified\":\"2026-03-10T12:24:09+00:00\",\"author\":{\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869\"},\"breadcrumb\":{\"@id\":\"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/#breadcrumb\"},\"inLanguage\":\"da-DK\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/longchangextracts.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/longchangextracts.com\/#website\",\"url\":\"https:\/\/longchangextracts.com\/\",\"name\":\"China Chemical Manufacturer\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/longchangextracts.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"da-DK\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869\",\"name\":\"longcha9\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"da-DK\",\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g\",\"caption\":\"longcha9\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor - China Chemical Manufacturer","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/longchangextracts.com\/da\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/","og_locale":"da_DK","og_type":"article","og_title":"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor - China Chemical Manufacturer","og_description":"Brefeldin A (BFA) is a powerful fungal metabolite that has become an indispensable tool in modern cell biology and cancer research. With the CAS registry number 20350-15-6, this macrocyclic lactone is produced by the fungus Penicillium brefeldianum and has captured the attention of researchers worldwide for its unique ability to interfere with fundamental cellular processes. […]","og_url":"https:\/\/longchangextracts.com\/da\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/","og_site_name":"China Chemical Manufacturer","article_published_time":"2026-03-10T12:24:09+00:00","author":"longcha9","twitter_card":"summary_large_image","twitter_misc":{"Skrevet af":"longcha9","Estimeret l\u00e6setid":"5 minutter"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/","url":"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/","name":"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor - China Chemical Manufacturer","isPartOf":{"@id":"https:\/\/longchangextracts.com\/#website"},"datePublished":"2026-03-10T12:24:09+00:00","dateModified":"2026-03-10T12:24:09+00:00","author":{"@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869"},"breadcrumb":{"@id":"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/#breadcrumb"},"inLanguage":"da-DK","potentialAction":[{"@type":"ReadAction","target":["https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/longchangextracts.com\/brefeldin-a-cas-20350-15-6-comprehensive-guide-protein-transport-inhibitor-2\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/longchangextracts.com\/"},{"@type":"ListItem","position":2,"name":"Brefeldin A (CAS 20350-15-6): A Comprehensive Guide to This Protein Transport Inhibitor"}]},{"@type":"WebSite","@id":"https:\/\/longchangextracts.com\/#website","url":"https:\/\/longchangextracts.com\/","name":"Producent af kemikalier i Kina","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/longchangextracts.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"da-DK"},{"@type":"Person","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869","name":"longcha9","image":{"@type":"ImageObject","inLanguage":"da-DK","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","caption":"longcha9"}}]}},"_links":{"self":[{"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/posts\/10658"}],"collection":[{"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/comments?post=10658"}],"version-history":[{"count":0,"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/posts\/10658\/revisions"}],"wp:attachment":[{"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/media?parent=10658"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/categories?post=10658"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longchangextracts.com\/da\/wp-json\/wp\/v2\/tags?post=10658"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}