Study on the Chemical Components of Chloroform and n-Butanol Parts in Wide Muscle Vine
Kuanjinteng is a dried vine stem of Tinosporae sinenisis (Lour.) Merr., a plant belonging to the family Menispermaceae. It is mainly distributed in Guangdong, Guangxi, Yunnan, and other regions, with a wide distribution and high yield, and has a long history of folk application. According to the National Compendium of Chinese Herbal Medicines, Kuan Jin Teng has a slightly bitter taste and a cool nature, and has the effects of dispelling wind, relieving pain, and relaxing muscles and activating collaterals. Kuanjinteng, also known as “Lezhe” in Tibetan medicine, is a commonly used medicinal herb in Tibetan medicine. It is good at dispelling wind and dampness, relaxing tendons and activating collaterals, and has significant therapeutic effects. The “Jingzhu Bencao” records that Kuanjinteng “treats the combination of diarrhea and red blood stasis, as well as bacon disease, rheumatism, and diarrhea. In clinical practice, Kuan Jin Teng is mainly used to treat orthopedic diseases such as rheumatoid arthritis, lumbar muscle strain, and traumatic injuries, indicating its great research value and development potential. Modern pharmacological research has shown that plants of the genus Gallbladder have various pharmacological activities such as anti-inflammatory, anti radiation, anti-tumor, antiviral, and immunosuppressive effects. However, current research on the chemical composition of this genus mainly focuses on two species: Hainan Gallbladder and Heart Leaf Gallbladder. As the vine stem of the Chinese green cow gall plant in the genus Gallbladder, Kuan Jin Teng has a long history of folk application and reliable therapeutic effects. However, the current development and utilization of Kuan Jin Teng is still relatively low, and the research on its pharmacological substance basis by domestic and foreign scholars is still relatively weak, mainly focusing on the separation of chemical components in the ethyl acetate part. Pharmacological research is mostly focused on the decoction or extract of Kuan Jin Teng, and its pharmacological substance basis has not been fully elucidated. In order to further enrich the chemical composition of Platycodon grandiflorus, various chromatographic separation techniques were used in this experiment to study the chemical composition of the chloroform and n-butanol extraction parts of Platycodon grandiflorus. The isolated compounds were also tested for their inhibition of lipopolysaccharide induced NO release activity in RAW264.7 cells, in order to search for and discover active ingredients from Platycodon grandiflorus and lay a foundation for further development and utilization.

 

In this experiment, multiple separation techniques were used to isolate and identify 13 compounds from the chloroform and n-butanol parts of the broad leaved vine, including 2 brain glycoside lipid compounds (1, 3), 5 phenylpropanoid compounds (5, 7, 10, 11, 12), 2 terpenoid compounds (4, 9), 1 amino acid compound (13), and 3 other compounds (2, 6, 8). Among them, compound 8 was reported for the first time as a natural product, compounds 1, 2, 3, 6, and 11 were isolated for the first time from plants in the family Menispermaceae, and compound 13 was isolated for the first time from Platycodon grandiflorus. There are research reports that compound 4 exhibits certain inhibitory activity on human oral epidermoid carcinoma KB cells and cervical squamous cell carcinoma SiHa cells; Compound 3 can alleviate synovial inflammation and prevent joint injury by reducing MCP-1 expression and inhibiting monocyte/macrophage infiltration into synovial cells. Based on the study of chemical composition, this article used LPS induced RAW264.7 cells as an in vitro inflammatory model to evaluate the anti-inflammatory activity of 13 isolated compounds. The results showed that compounds 2, 3, and 4 had certain anti-inflammatory activity by inhibiting NO production experiments. This study not only enriches the chemical structure types and chemical composition library of Platycodon grandiflorus, but also provides a scientific basis for further elucidating its pharmacological substance basis and promoting the resource development of Platycodon grandiflorus.