Study on the Chemical Composition of Tianshan Fake Wolf Venom
Wolf venom was first recorded in the “Shennong Bencao Jing”, with a mild nature, bitter and pungent taste, and high toxicity; It has the effects of removing phlegm, reducing accumulation, and relieving pain. It is mainly used to treat edema, bloating, phlegm and insect accumulation, abdominal pain, chronic bronchitis, lymph node tuberculosis, sores and ringworm, etc. However, in clinical use, it is very confusing and there are many counterfeit products. The authentic product is wolfberry. Stelleropsis tianschanica, a plant of the genus Stelleropsis in the family Ericaceae, has a certain phylogenetic relationship with Stelleropsis tianschanica in terms of species, and shares many common components. It is commonly used as a counterfeit of traditional Chinese medicine wolf venom in Xinjiang; It is 15-30cm high, with a head shaped inflorescence or extremely short spike shaped inflorescence, woody rhizome, yellow brown or light brown in color; Growing in mountainous grasslands at an altitude of 1700-2000m, it is only distributed in Zhaosu County, Xinjiang, China and Kyrgyzstan abroad. Due to its unique geographical environment and growth area, there is a lack of research on the functional components of Tianshan fake wolfberry. The compounds currently isolated from it mainly include lignans, flavonoids, terpenes, phenols, and other chemical components, but research reports on their pharmacological aspects are still blank. Therefore, in order to further study the material basis of Tianshan fake wolfberry, this experiment conducted chemical composition research on it and tested the cytotoxic activity of the isolated compounds, providing reference for the development and utilization of Tianshan fake wolfberry.

 

Through the extraction and isolation of Tian Shan fake wolfberry, a total of 15 compounds were obtained, among which 1-7, 12-15 were isolated from plants of the fake wolfberry genus for the first time. The anti-tumor activity of the 15 compounds was screened, and the results showed that compounds 1, 2, 3, and 10 had certain inhibitory activity on HGC-27 gastric cancer cells. Among them, 1, 2, and 10 are diterpenoid compounds of the rosmarine type, indicating that these compounds can be used as potential anti-tumor drugs in fake wolfberry venom. Due to the fact that Tian Shan fake wolfberry is used as a confused or counterfeit wolfberry in remote areas of Xinjiang, but there are not many research literature reports on it at home and abroad, and the research on its therapeutic mechanism is relatively insufficient, we have further enriched the chemical composition library and anti-tumor activity of Tian Shan fake wolfberry through research, providing a theoretical basis for better development and utilization of this plant.