Study on the anti-tumor activity of chemical components of the lipid family in Lycopodium serratum Thunb, Also known as snake foot cypress or snake foot grass, it is a plant in the family Taxaceae, usually 10-30cm tall, with pale yellow kidney shaped sporangia visible on both sides of the spore leaves. The Thousand Layer Pagoda is mainly distributed in southern China and other Asian countries (such as Japan, Thailand, the Philippines, and Malaysia), growing in forests or shrubs at an altitude of 300-2700m. In Chinese folk medicine, the use of the Thousand Layer Pagoda has a history of more than a thousand years, and its chemical components mainly include lycorine alkaloids and triterpenes. In clinical practice, alkaloids have been successfully used to treat myasthenia gravis, Alzheimer’s disease, and dementia.

Natural products have structural diversity and novelty, and are an important source of drug lead molecules. The main structural types of chemical components in this plant include alkaloids, triterpenoids, and aliphatic compounds. Alkaloids exhibit acetylcholinesterase inhibitory activity, while triterpenoids exhibit anti-tumor activity. However, there are few reports on the biological activity of their aliphatic compounds.

Kanglaite Injection is a drug used to treat spleen deficiency, small cell lung cancer, lung cancer, primary liver cancer, and liver cancer. Its active ingredients include triglycerides, diglycerides, monoglycerides, and fatty acid esters, among other fatty acid compounds. The aim of this study is to enrich, separate, and purify aliphatic compounds in a thousand layer tower, and to screen the anti-tumor activity of the isolated monomer compounds. In the preliminary experiments, we preliminarily found that the chloroform layer extracted from the whole plant of the Thousand Layer Pagoda has the biological activity of inhibiting HepG2 cells. Combining various modern separation and purification techniques, a systematic separation of chemical components in the thousand layer tower is carried out. The separated chemical components are characterized by spectroscopic methods, and the isolated monomer compounds are tested for HepG2 cell inhibitory activity, in order to provide reference for the further development and utilization of the thousand layer tower.

 

 

This study extracted the entire medicinal plant of the Thousand Layer Pagoda by acid extraction and alkaline precipitation to obtain the extract solution. After extraction, the active site chloroform layer was separated and purified. A total of 9 aliphatic compounds were isolated and identified, among which compounds 1 and 2 were new compounds. Compounds 3-9 were isolated from the Thousand Layer Pagoda for the first time, and the isolated monomeric compounds were tested for in vitro inhibition of HepG2 cell activity.
The fatty compounds in the Thousand Layer Tower have not been studied by scholars, and this experiment reports for the first time nine fatty compounds in the Thousand Layer Tower. In the preliminary pharmacological activity experiments, it was found that the chloroform extraction layer of the crude extract of the Thousand Layer Tower had the effect of inhibiting the activity of HepG2 cells. A systematic separation and purification were carried out on it, and the screening of the biological activity of aliphatic compounds benefited from the inspiration of Kanglaite drugs. The active ingredient of Kanglaite is coix seed oil, mainly composed of triglycerides. Therefore, we screened the anti HepG2 cell activity of the 9 isolated adipogenic compounds, and the results showed that compounds 1 and 3 had inhibitory effects on HepG2 cells, with IC50 values of 41.12 and 80.52 μ mol/L, respectively. This study, for the first time, focused on the isolation and identification of aliphatic compounds in the Thousand Layer Pagoda, which enriched the chemical composition of the Thousand Layer Pagoda to a certain extent. In addition, this experiment conducted for the first time the study on the inhibitory activity of monomers from the Thousand Layer Pagoda on HepG2 cells, providing a reference for the rational development and utilization of the plant chemical resources of the Thousand Layer Pagoda, and laying the foundation for the pharmacological research of the Thousand Layer Pagoda.